Immunological detection method of pyrethroid compound, haptenic compound, intermediate for the haptenic compound, immunogen and antibody
申请人:MITSUI TOATSU CHEMICALS, Inc.
公开号:EP0645628A1
公开(公告)日:1995-03-29
Described is a detection method of a pyrethroid compound which comprises providing a first antibody against the pyrethroid compound, said first antibody having been obtained by immunizing a responder with an immunogen which is a first conjugate between a first carrier and a first haptenic compound represented by the following formula (1) or (2):
mixing the first antibody with the sample solution,
causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; and
reacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith.
This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.
描述了一种拟除虫菊酯化合物的检测方法,该方法包括提供针对拟除虫菊酯化合物的第一抗体,所述第一抗体是通过用免疫原免疫应答者而获得的,免疫原是第一载体和由下式(1)或(2)代表的第一合酶化合物之间的第一共轭物:
将第一抗体与样品溶液混合、
使第一抗体的任何未反应部分作为包被抗原结合到第二共轭物上,所述第二共轭物由第二载体和第二合酶化合物组成,并包被在固相载体表面,从而获得抗原-抗体共轭物;以及
将抗原-抗体共轭物与针对第一抗体的第二抗体反应,所述第二抗体具有与之相连的标记酶。
本发明使得对我们周围环境(如水或土壤、植物或食物)中的拟除虫菊酯化合物进行简便、经济和快速的分析成为可能。