2,6-dihydroxybenzhydroxamic acid | 35318-17-3

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 2,6-dihydroxybenzhydroxamic acid
• 英文别名: 2,6-Dihydroxybenzohydroxamic Acid；N,2,6-trihydroxybenzamide
• CAS: 35318-17-3
• 化学式: C₇H₇NO₄
• 分子量: 169.137
• InChiKey: VBPSEOFCRWRCKY-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.5
• 重原子数：
  12
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  89.8
• 氢给体数：
  4
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  2,6-二羟基苯甲酸甲酯 生成 2,6-dihydroxybenzhydroxamic acid
  参考文献：
  名称：

  VANT, RIET, B.;ELFORD, H. L.;WAMPLER, G. L.

  摘要：

  DOI：

文献信息

• Methods for the preparation of chemically misaminoacylated tRNA via protective groups
  申请人：AMBERGEN, INC.

  公开号：US20030219780A1

  公开（公告）日：2003-11-27

  The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.

  本发明涉及用于制备化学氨酰化tRNA的方法，目的是将标记物引入新生蛋白质中。本发明还涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白质的非放射性标记、检测、定量和分离的方法，利用化学氨酰化的tRNA。tRNA分子被非放射性标记物误氨酰化，这些标记物可以是非天然氨基酸、氨基酸类似物或衍生物。标记物可能包括可切割的基团、可检测的标签、报告性质，其中蛋白质中包含的标记物可以与未包含的标记物区分开来，或者促进检测和从翻译系统的其他组分中分离新生蛋白质的耦合剂。
• Thermodynamic and structural study of complexation of phenylboronic acid with salicylhydroxamic acid and related ligands
  作者：Mayte A. Martínez-Aguirre、Marcos Flores-Alamo、Anatoly K. Yatsimirsky

  DOI：10.1002/aoc.4405

  日期：2018.8

  Stability constants of boronate complexes with a highly efficient bioconjugation ligand salicylhydroxamic acid, its derivatives and some structurally related compounds were determined by potentiometric and spectroscopic titrations at variable pH allowing one to obtain detailed stability – pH profiles and to identify the optimum pH for complexation with each ligand. The N,O‐binding of salicylhydroxamic

  硼酸盐与高效生物共轭配体水杨基异羟肟酸的配合物，其衍生物和一些与结构相关的化合物的稳定性常数通过电位计和分光光度滴定法在不同的pH值下确定，从而获得详细的稳定性– pH曲线并确定与每种络合的最佳pH配体。水杨酸异羟肟酸通过硼酸与酚OH和异羟肟酸NH基团的缩合形成N，O-结合，是通过分离的苯基硼酸和4-硝基苯基硼酸配合物的晶体结构确定的。尽管对于N-甲基化水杨基异羟肟酸而言，这种结合是不可能的，但它仍会形成稳定的硼酸酯络合物，据称其涉及异常的7元–O–B–O–循环，并受11 H NMR研究。缺少邻-OH基团的异羟肟酸和水杨酰酰肼形成的硼酸酯络合物较不稳定，但其稳定性与消泡剂络合物相似，可用于共轭应用。与其他形成四面体阴离子络合物的配体相反，水杨酰胺肟形成四面体但在弱酸溶液中具有高稳定性的中性硼酸酯络合物。2,6-二羟基苯并异羟肟酸在中性和酸性溶液中的亲和力最高，超过了水杨基异羟肟酸（K obs
• PURIFICATION OF PRIMER EXTENSION PRODUCTS
  申请人：——

  公开号：US20010049438A1

  公开（公告）日：2001-12-06

  This invention provides methods for purifying nucleic acids, in particular primer extension products such as those obtained in nucleic acid sequencing reactions. The methods involve the use of a primer to which is attached a string of arylboronic acid moieties. After extension of the primer using a polymerase, the primer extension products are complexed with a solid support to which is attached an arylboronic acid complexing moiety. The resulting complex is separated from the reaction mixture, washed, and the primer extension products are dissociated from the solid support. The primer extension products are obtained in a form particularly suitable for loading directly on a capillary electrophoresis apparatus.

  本发明提供了一种纯化核酸的方法，特别是用于纳米级核酸测序反应中获得的引物延伸产物。该方法涉及使用一个引物，该引物附有一串芳基硼酸基团。在使用聚合酶延伸引物后，将引物延伸产物与固定有芳基硼酸络合基团的固体支持物复合。然后将所得的复合物从反应混合物中分离，清洗，并使引物延伸产物从固体支持物中解离。引物延伸产物以特别适合直接加载在毛细管电泳设备上的形式获得。
• 1,2-phenylenediboronic acid reagents and complexes
  申请人：——

  公开号：US20020038004A1

  公开（公告）日：2002-03-28

  A reagent having the general formula of General Formula I: 1 wherein group Z comprises a spacer selected from an aliphatic chain up to about 6 carbon equivalents in length, an unbranched aliphatic chain of from about 6 to 18 carbon equivalents in length with at least one of an intermediate amide and a disulfide moiety, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length; wherein group R is an electrophilic or nucleophilic moiety suitable for reaction of the reagent with a biologically active species; and wherein group Q is one of nothing at all, an amide, a methyl amide, a methylene, an ether, a thioether, a methyl ether, and a methyl thioether moiety. Also, a conjugate, a bioconjugate and a method of conjugating.

  一种具有通式I的试剂：其中Z基包括从长达约6个碳当量的脂肪链、从大约6到18个碳当量的直链脂肪链，其中至少有一个中间酰胺和二硫键基团，以及从大约3到12个碳当量的聚乙二醇链；其中R基是适用于试剂与生物活性物种反应的亲电或亲核基团；而Q基是无、酰胺、甲基酰胺、亚甲基、醚、硫醚、甲基醚和甲基硫醚基团之一。此外，还提供了一种共轭物、生物共轭物和共轭方法。
• Bifunctional boronic compound complexing reagents and complexes
  申请人：——

  公开号：US20030105280A1

  公开（公告）日：2003-06-05

  A reagent having the general formula of General Formula I: 1 wherein group R is an electrophilic or a nucleophilic moiety suitable for reaction of the reagent with a biologically active species; group R 2 is one of H and OH moieties; group R 3 is one of an alkyl and a methylene bearing an electronegative substituent; group Z is one of (CH 2 ) n and CH 2 O(CH 2 CH 2 O) n 2 , and n is an integer of from 1 to 5, and n 2 is an integer of from 1 to 4; and each of group Z 2 and Z 3 are (CH 2 )CONH(CH 2 ) 3 CONH(CH 2 ) 2 . Also, a reagent having the general formula of General Formula II: 2 group R 2 is one of H and OH moieties; group Z is one of (CH 2 ) n and CH 2 O(CH 2 CH 2 O) n 2 , and n is an integer of from 1 to 5, and n 2 is an integer of from 1 to 4; each of group Z 2 and group Z 3 include one of (CH 2 )CONH(CH 2 ) 3 CONH(CH 2 ) 2 ; and (CH 2 )CONH(CH 2 ); and group R is an electrophilic or a nucleophilic moiety suitable for reaction of the reagent with the biologically active species.

  一种具有通式I的试剂：其中R基团是适合与生物活性物种反应的亲电性或亲核性官能团；R2基团是H和OH官能团之一；R3基团是烷基或带有电负取代基的亚甲基；Z基团是(CH2)n和CH2O(CH2CH2O)n2之一，其中n是1至5的整数，n2是1至4的整数；Z2和Z3基团均为(CH2)CONH(CH2)3CONH(CH2)2。另外，一种具有通式II的试剂：其中R2基团是H和OH官能团之一；Z基团是(CH2)n和CH2O(CH2CH2O)n2之一，其中n是1至5的整数，n2是1至4的整数；Z2和Z3基团均包括(CH2)CONH(CH2)3CONH(CH2)2和(CH2)CONH(CH2)之一；而R基团是适合与生物活性物种反应的亲电性或亲核性官能团。