[[5-[5-[(E)-3-aminoprop-1-enyl]-2,4-dioxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: [[5-[5-[(E)-3-aminoprop-1-enyl]-2,4-dioxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• 化学式: C₁₂H₂₀N₃O₁₄P₃
• 分子量: 523.22
• InChiKey: AAQWIRIAZPJBAM-OWOJBTEDSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -8.2
• 重原子数：
  32
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  265
• 氢给体数：
  7
• 氢受体数：
  15

文献信息

• System for delivering therapeutic agents into living cells and cells nuclei
  申请人：Segev David

  公开号：US20080004234A1

  公开（公告）日：2008-01-03

  A novel class of oligomeric compounds designed for forming conjugates with biologically active substances and delivering these substances to a desired bodily target are disclosed. Novel conjugates of these oligomeric compounds and biologically active moieties, pharmaceutical compositions containing such conjugates, and uses thereof as delivery systems for delivering the biologically active substances to a desired target are further disclosed. Processes of preparing the conjugates and the oligomeric compounds and novel intermediates designed for and used in these processes are also disclosed.

  本发明揭示了一类新型的寡聚化合物，旨在与生物活性物质形成共轭物，并将这些物质传递到所需的身体靶点。本发明还揭示了这些寡聚化合物与生物活性物质的新型共轭物，含有这种共轭物的制药组合物，以及将其用作传递生物活性物质到所需目标的传递系统的用途。本发明还揭示了制备这些共轭物和寡聚化合物的过程以及设计和用于这些过程的新型中间体。
• Enzymatic nucleic acid synthesis
  申请人：President and Fellows of Harvard College

  公开号：US10745814B2

  公开（公告）日：2020-08-18

  The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.

  本公开提供了活化酶的方法，如易出错或与模板无关的聚合酶，利用电能改变反应区和反应位点的 pH 值，从酶无活性的失活 pH 值变为酶有活性的活化 pH 值，从而将核苷酸添加到引发剂或生长的聚合物链中。然后可将激活 pH 值变回失活 pH 值，并根据需要多次重复该过程，以产生目标核酸序列。
• COMPOSITIONS, METHODS AND SYSTEMS FOR SINGLE MOLECULE SEQUENCING
  申请人：Hardin Susan H.

  公开号：US20110165652A1

  公开（公告）日：2011-07-07

  Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.
• Enzymatic Nucleic Acid Synthesis
  申请人：President and Fellows of Harvard College

  公开号：US20190112626A1

  公开（公告）日：2019-04-18

  The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3′ terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3′ terminal nucleotide such that the selected nucleotide becomes a 3′ terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
• US8680062B2
  申请人：——

  公开号：US8680062B2

  公开（公告）日：2014-03-25