2H-Furo(2,3-h)-1-benzopyran-2-one, 8,9-dimethyl- | 78982-41-9

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 2H-Furo(2,3-h)-1-benzopyran-2-one, 8,9-dimethyl-
• 英文别名: 8,9-dimethylfuro[2,3-h]chromen-2-one
• CAS: 78982-41-9
• 化学式: C₁₃H₁₀O₃
• 分子量: 214.22
• InChiKey: JLAPWSNBHNHNRQ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.1
• 重原子数：
  16
• 可旋转键数：
  0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.15
• 拓扑面积：
  39.4
• 氢给体数：
  0
• 氢受体数：
  3

文献信息

• Immobilized nucleic acid probe and solid support for nucleic acids
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0130523A2

  公开（公告）日：1985-01-09

  A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol-diglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.

  一种经适当辐照后能与核酸结合的固体支持物，包括(a)固体基底，(b)光化学反应性插层化合物或其他核酸结合配体，以及(c)将基底和配体(b)化学连接的二价自由基。具体来说，含羟基的固体基质（如硝酸纤维素纸）通过双功能试剂（如溴化氰或 1,4-丁二醇二缩水甘油醚）与氨基取代的当归苷或补骨脂素或溴化乙锭连接，而后者又与核酸进行光化学连接。由此产生的固定化核酸探针能够与互补核酸片段杂交，因此可用于诊断检测。
• Photochemical method of labelling nucleic acids for detection in hybridization assays
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0187332A2

  公开（公告）日：1986-07-16

  In a test for the presence of a particular nucleic acid in a test sample wherein the same is subjected to hybridizing conditions with a Ibeled nucleic acid probe, and the product is assayed for the presence of said label, the improvement wherein said probe comprises a probe according to claim 1. A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemical- ly linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c).

  2.一种检测试样中是否存在特定核酸的方法，将试样与标记的核酸探针在杂交条件下进行杂交，检测产物中是否存在所述标记，其中所述探针包括根据权利要求1所述的探针。 3.一种标记的核酸探针，包括(a)核酸成分，(b)与核酸成分光化学连接的核酸结合配体，(c)标记和(d)化学连接(b)和(c)的间隔物。
• Rapid detection of nucleic acid sequences in a sample by labeling the sample
  申请人：MILES INC.

  公开号：EP0235726A2

  公开（公告）日：1987-09-09

  A method for detecting one or more microorganisms or polynucleotide sequences from eukaroytic sources in a nucleic acid-containing test sample comprising (a) preparing a test sample comprising labeling the nucleic acids in the test sample, (b) preparing one or more process by immobilizing a single-stranded nucleic acid of one or more known microorganisms or sequences from eukaroytic sources, (c) contacting, under hybridization conditions, the labeled single-stranded nucleic acid to form hybridized labeled nucleic acids, and (d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.

  一种检测含核酸检测样本中一种或多种微生物或真核生物来源的多核苷酸序列的方法，包括 (a) 制备测试样品，包括标记测试样品中的核酸、 (b) 通过固定一种或多种已知微生物的单链核酸或真核来源的核酸序列，制备一 个或多个过程、 (c) 在杂交条件下，与标记的单链核酸接触，形成杂交标记核酸，以及 (d) 通过检测标记来检测杂交核酸。该方法可用于检测遗传疾病，如镰状细胞性贫血。
• Solution-phase hybridization assay for detecting polynucleotide sequences
  申请人：MILES INC.

  公开号：EP0237833B1

  公开（公告）日：1993-01-13
• US4968602A
  申请人：——

  公开号：US4968602A

  公开（公告）日：1990-11-06