申请人:TAIWAN SUGAR CORPORATION
公开号:US20150226671A1
公开(公告)日:2015-08-13
A method for determining the activity of a nucleic-acid-repair enzyme is provided. The method comprises the following steps: (i) providing a double-stranded nucleic acid molecule, which is labeled with a fluorophore and a quencher and has at least one mutated nucleotide in either strand; (ii) mixing the double-stranded nucleic acid molecule, S1 nuclease, and the sample to obtain a mixture; and measuring the fluorescence intensity of the mixture.