申请人:Erbacher Christoph
公开号:US20110319506A1
公开(公告)日:2011-12-29
The present invention relates to a method for purifying nucleic acids using a nucleic acid-binding phase which is furnished with a deficit of nucleic acid-binding groups A having a pK of 8 to 13, or which has groups A and binding-inhibiting groups N which are neutrally charged during the binding, and preferably during the elution, and the method comprises the following steps: (a) binding the nucleic acids to the nucleic acid-binding phase at a pH which is below the pH of the pK of the nucleic acid-binding groups A (binding pH); (b) eluting the nucleic acids at a pH which is above the binding pH (elution pH). In addition, corresponding kits and also nucleic acid-binding phases which can be used for purifying nucleic acids are disclosed. The technology according to the invention permits the purification of nucleic acids and, in particular, elution, with use of low salt concentrations, and so the purified nucleic acids can be directly processed, for example used in a PCR.
本发明涉及一种利用具有pK值为8至13的核酸结合基团A的核酸结合相或具有在结合期间和最好在洗脱期间呈中性电荷的基团A和结合抑制基团N的核酸结合相来纯化核酸的方法,该方法包括以下步骤:(a)在低于核酸结合基团A的pK值的pH下(结合pH)将核酸结合到核酸结合相上;(b)在高于结合pH的pH下(洗脱pH)洗脱核酸。此外,还公开了相应的用于纯化核酸的试剂盒和核酸结合相。根据本发明的技术允许纯化核酸,特别是在低盐浓度下进行洗脱,因此纯化的核酸可以直接处理,例如用于PCR。