共轭皮质醇-氨基戊基乙基异发光氨 | 76773-85-8

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 类固醇和类固醇衍生物
• 中文名称: 共轭皮质醇-氨基戊基乙基异发光氨
• 英文名称: cortisol-aminopentyl ethyl isoluminol conjugate
• 英文别名: Cortisol-aminopentylethylisoluminol conjugate；[2-[(8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-10,13-dimethyl-3-oxo-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-17-yl]-2-oxoethyl] 4-[5-[(1,4-dioxo-2,3-dihydrophthalazin-6-yl)-ethylamino]pentylamino]-4-oxobutanoate
• CAS: 76773-85-8
• 化学式: C₄₀H₅₄N₄O₉
• 分子量: 734.89
• InChiKey: ARPVYTBUQVLNPO-RHJPVIBVSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.3
• 重原子数：
  53
• 可旋转键数：
  15
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.65
• 拓扑面积：
  191
• 氢给体数：
  5
• 氢受体数：
  10

反应信息

• 作为产物：
  描述：

  氢化可的松琥珀酸酯 、 6--amino-2,3-dihydrophthalazine-1,4-dione 在 N-羟基丁二酰亚胺 、 聚碳化二亚胺 作用下， 生成 共轭皮质醇-氨基戊基乙基异发光氨
  参考文献：
  名称：

  An immunoassay for plasma cortisol based on chemiluminescence

  摘要：

  An immunoassay procedure for the determination of cortisol in human plasma is described, which utilizes chemiluminescence as the end point. A cortisol-isoluminol conjugate serves as the chemiluminescent marker. The light emission by this conjugate upon oxidation is delayed by prior incubation with anti-cortisol IgG, but not by unrelated gamma-globulin. This delayed light emission was inhibited by cortisol in a dose-dependent manner, with a linear range of 20-1000 pg steroid/assay tube. A competitive protein binding assay based on this procedure was applied to methylene chloride extracts of cortisol from normal and pathological human plasma (2-40 micrograms/100 ml). Cortisol values obtained by this procedure agreed well with those obtained by radioimmunoassay, using the same antiserum with tritiated cortisol as the label (r = 0.98). The chemiluminescence immunoassay is comparable to radioimmunoassay with regard to sensitivity, specificity, precision and accuracy. The advantage of the new assay procedure is that it obviates the need for counting radioactivity and for separation of bound and free ligand.

  DOI：

  10.1016/0039-128x(80)90030-6