CELL TRAPPING METHOD, METHOD FOR PRODUCING SPECIFIC CELL-TRAPPING DEVICE, AND METHOD FOR PRODUCING SPECIFIC CELL-CONTAINING SOLUTION
申请人:Hitachi Chemical Company, Ltd.
公开号:EP3187578A1
公开(公告)日:2017-07-05
Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device includes a housing which has an introduction region for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior; and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.
本发明提供了一种细胞捕获方法,用于通过使用细胞捕获装置过滤液体,在过滤表面选择性地捕获包含在含细胞溶液中的特定细胞,细胞捕获装置包括壳体,该壳体具有引入区,用于将选自包含两种或多种细胞的含细胞溶液的液体和用于处理含细胞溶液中的细胞的处理液引入内部,以及排出流路,用于将液体排出到外部;以及一个过滤器,该过滤器设置在导入区域和排出流路之间的流路上,并且在厚度方向上形成多个供液体流过的通孔,该方法包括一个排液步骤,该步骤将液体排出n次(n为自然数),其中从第一步排液到第n步排液、在过滤器上的导入区域中,液体的液面保持在预定的液面高度,当第 n 次排液步骤完成时,在过滤器中液面处于预定高度的状态下,停止从电池捕集装置中排出液体。