2'-脱氧-5-羟基尿苷磷酸酯(1:3) | 27972-89-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 2'-脱氧-5-羟基尿苷磷酸酯(1:3)
• 中文别名: 5-羟基-1-[(2R,4S,5R)-4-羟基-5-(羟甲基)四氢呋喃-2-基]嘧啶-2,4-二酮磷酸
• 英文名称: 5-Hydroxydeoxyuridine triphosphate
• 英文别名: [(2R,3S,5R)-3-hydroxy-5-(5-hydroxy-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methyl [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate
• CAS: 27972-89-0
• 化学式: C₉H₁₅N₂O₁₅P₃
• 分子量: 484.14
• InChiKey: RLYLTZXYBNQTOL-UBKIQSJTSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.5
• 重原子数：
  29
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  259
• 氢给体数：
  7
• 氢受体数：
  15

文献信息

• Bioagent detection oligonucleotides
  申请人：IBIS BIOSCIENCES, INC.

  公开号：US10662485B2

  公开（公告）日：2020-05-26

  The present invention compositions, methods and systems to identify, detect, and/or quantify bacterial DNA in the presence of contaminating non-bacterial DNA. In particular, the present invention provides oligonucleotides configured to detect a relatively small amount of bacterial DNA in the presence of an overwhelmingly large amount of contaminating human DNA.

  本发明的组合物、方法和系统可在有非细菌 DNA 污染的情况下识别、检测和/或量化细菌 DNA。特别是，本发明提供的寡核苷酸可在大量人类 DNA 污染的情况下检测相对少量的细菌 DNA。
• Sequencing using non-natural nucleotides
  申请人：Ultima Genomics, Inc.

  公开号：US11326207B2

  公开（公告）日：2022-05-10

  The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

  本公开提供了核酸测序的方法和系统。这些系统和方法可实现与上下文无关的整合，降低上下文依赖性，或具有可进行校准和建模的上下文依赖性。这些系统和方法还可以减少误结合。
• Partially double-stranded nucleic acids, methods of making, and use thereof
  申请人：——

  公开号：US20020127575A1

  公开（公告）日：2002-09-12

  A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a terminal end. The process comprises the steps of (a) providing at least one primer, P 1 , containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P 1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P 1 ; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer P 1 from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase.

  本发明公开了一种用于产生至少一种部分双链目标核酸的工艺，该核酸的末端含有至少一个单链区。该工艺包括以下步骤 (a) 提供至少一种引物 P 1 (b) 将至少一个靶核酸序列与 P 1 生成含有至少一个易损核苷酸的双链多核苷酸；(c) 将双链多核苷酸暴露于可促进多核苷酸在引物 P 1 的至少一个易损核苷酸位点处发生单链裂解的条件下。 1 和 (d) 将裂解的多核苷酸暴露于促进引物 P 1 从末端解离的条件。可变核苷酸可以是 dUTP，其中多核苷酸在可变核苷酸位点的单链裂解是通过尿嘧啶 N-糖基化酶处理进行的。
• Fluorescence-based genotyping
  申请人：——

  公开号：US20030165880A1

  公开（公告）日：2003-09-04

  The present invention relates to a method for genotyping a diploid organism to detect single nucleotide polymorphisms (SNPs) using modified nucleotides or nucleotide residues substituted with fluorescent groups.

  本发明涉及一种利用经修饰的核苷酸或被荧光基团取代的核苷酸残基对二倍体生物进行基因分型以检测单核苷酸多态性（SNPs）的方法。
• Compositions for use in identification of adventitious viruses
  申请人：Sampath Rangarajan

  公开号：US20060205040A1

  公开（公告）日：2006-09-14

  The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis.

  本发明提供了通过分子质量和碱基组成分析快速鉴定和定量不定期污染病毒的组合物、试剂盒和方法。