azido-Rh110-t-Boc | 1027627-68-4

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: azido-Rh110-t-Boc
• 英文别名: tert-butyl N-(6'-azido-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl)carbamate
• CAS: 1027627-68-4
• 化学式: C₂₅H₂₀N₄O₅
• 分子量: 456.458
• InChiKey: HSOFFXPFRQLPEY-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.6
• 重原子数：
  34
• 可旋转键数：
  4
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  88.2
• 氢给体数：
  1
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  azido-Rh110-t-Boc 在 盐酸 作用下， 以 1,4-二氧六环 为溶剂， 反应 2.5h， 以99%的产率得到3-amino-6-azidoxanthene-9'-spiro-1'-isobenzofuran-3'(1'H)-one
  参考文献：
  名称：

  EP2103616

  摘要：

  公开号：
• 作为产物：
  描述：

  叔-丁基氯甲酸酯 在 sodium hydride 、 三氟乙酸 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 、 乙腈 为溶剂， 反应 3.0h， 生成 azido-Rh110-t-Boc
  参考文献：
  名称：

  Reaction-Based Fluorescent Probes for Selective Imaging of Hydrogen Sulfide in Living Cells

  摘要：

  Hydrogen sulfide (H2S) is emerging as an important mediator of human physiology and pathology but remains difficult to study, in large part because of the lack of methods for selective monitoring of this small signaling molecule in live biological specimens. We now report a pair of new reaction-based fluorescent probes for selective imaging of H2S in living cells that exploit the H2S-mediated reduction of azides to fluorescent amines. Sulfidefluor-1 (SF1) and Sulfidefluor-2 (SF2) respond to H2S by a turn-on fluorescence signal enhancement and display high selectivity for H2S over other biologically relevant reactive sulfur, oxygen, and nitrogen species. In addition, SF1 and SF2 can be used to detect H2S in both water and live cells, providing a potentially powerful approach for probing H2S chemistry in biological systems.

  DOI：

  10.1021/ja203661j

文献信息

• FLUORESCENT PROBES FOR REACTIVE SULFUR SPECIES
  申请人：CHANG Christopher J.

  公开号：US20120329085A1

  公开（公告）日：2012-12-27

  The invention provides reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells.

  本发明提供了一种基于反应的荧光探针，用于活细胞中硫化氢的选择性成像。
• Triphenylphosphinecarboxamide: An Effective Reagent for the Reduction of Azides and Its Application to Nucleic Acid Detection
  作者：Hisao Saneyoshi、Tatsuya Ochikubo、Takushi Mashimo、Ken Hatano、Yoshihiro Ito、Hiroshi Abe

  DOI：10.1021/ol402832w

  日期：2014.1.3

  designed and synthesized as alternative reagents to triphenylphosphine for the facile reduction of azides. The TPPc derivatives performed as efficient reducing agents for the synthesis of primary amines without the need for an additional hydrolysis procedure. The TPPc derivatives were also applied to nucleic acid sensing using a RhAz-oligonucleotide conjugate in a DNA-templated fluorogenic reaction.

  设计并合成了一系列三苯基膦羧酰胺（TPPc）衍生物，作为三苯基膦的替代试剂，可轻松还原叠氮化物。TPPc衍生物可作为合成伯胺的有效还原剂，而无需其他水解程序。TPPc衍生物还用于在DNA模板荧光反应中使用RhAz-寡核苷酸共轭物进行核酸传感。
• FLUORESCENCE-PRODUCING MOLECULE
  申请人：Abe Hiroshi

  公开号：US20100190159A1

  公开（公告）日：2010-07-29

  It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R 1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R 2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R 3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R 4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R 3 may bind to R 4 to form a ring.
• US8426127B2
  申请人：——

  公开号：US8426127B2

  公开（公告）日：2013-04-23
• EP2103616
  申请人：——

  公开号：——

  公开（公告）日：——