PhCO-Asn-Leu-Thr-NH2 | 87980-77-6

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: PhCO-Asn-Leu-Thr-NH2
• 英文别名: Bz-Asn-Leu-Thr-NH2；(2S)-N-[(2S)-1-[[(2S,3R)-1-amino-3-hydroxy-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]-2-benzamidobutanediamide
• CAS: 87980-77-6
• 化学式: C₂₁H₃₁N₅O₆
• 分子量: 449.507
• InChiKey: KDQJFVBRUWTMKD-DYWXZXKOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.8
• 重原子数：
  32
• 可旋转键数：
  12
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.48
• 拓扑面积：
  194
• 氢给体数：
  6
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  H-Asn-Leu-Thr-NH2 生成 PhCO-Asn-Leu-Thr-NH2
  参考文献：
  名称：

  CLARK, RICHARD S.;BANERJEE, SHYAMA;COWARD, JAMES K., J. ORG. CHEM., 55,(1990) N6, C. 6275-6285

  摘要：

  DOI：

文献信息

• Yeast oligosaccharyltransferase: glycosylation of peptide substrates and chemical characterization of the glycopeptide product
  作者：Richard S. Clark、Shyamal Banerjee、James K. Coward

  DOI：10.1021/jo00313a013

  日期：1990.12

  The product of the reaction catalyzed by yeast oligosaccharyltransferase was examined in order to determine the nature of the chemical linkage between the sugar and peptide. Biosynthetic donor lipid [H-3]oligosaccharide was prepared and used as a substrate for yeast oligosaccharyltransferase together with a chemically synthesized peptide acceptor, N-benzoyl-Asn-Leu-Thr-NH2. the glycosylated peptide product of the in vitro reaction was isolated and hydrolyzed with endo-beta-N-acetylglucosaminidase-H to yield a large oligosaccharide and the glycotripeptide, N-benzoyl-Asn(GlcNAc)-Leu-Thr-Nh2. This glycopeptide was purified using gel filtration, affinity binding, and reverse-phase high-performance liquid chromatography. The biosynthetic glycopeptide was compared with chemically synthesized glycopeptides in which a 1-amino-GlcNAc moiety was linked to either the alpha- or beta-carboxyl of aspartate. It was determined that the sole biosynthetic product has the structure in which the carbohydrate is linked to the peptide through the beta-carbonyl of asparagine, i.e., a normal alpha-peptide. These experiments provide an unambiguous structural proof of the protein-carbohydrate linkage in the glycoprotein product of the oligosaccharyltransferase-catalyzed reaction.
• CLARK, RICHARD S.;BANERJEE, SHYAMA;COWARD, JAMES K., J. ORG. CHEM., 55,(1990) N6, C. 6275-6285
  作者：CLARK, RICHARD S.、BANERJEE, SHYAMA、COWARD, JAMES K.

  DOI：——

  日期：——
• [EN] PROTEIN COMPLEXES AND METHODS FOR THEIR USE<br/>[FR] COMPLEXES DE PROTEINES ET LEURS PROCEDES D'UTILISATION
  申请人：CELLZOME AG

  公开号：WO2003072602A2

  公开（公告）日：2003-09-04

  The present invention relates to multiprotein complexes from eukaryotes. The complexes are obtainable by using a protein as a bait and isolating the set of proteins which is attached thereto from cells. Such protein complexes may comprise up to 30 distinct proteins.