2-环戊烯-1-羧酸,3-异丙基-1-甲基-(5CI) | 84445-38-5

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 2-环戊烯-1-羧酸,3-异丙基-1-甲基-(5CI)
• 中文别名: 2',3'-二脱氧尿苷-5'-三磷酸酯
• 英文名称: Deoxyuridine triphosphate
• 英文别名: ddUTP；[[(2S,5R)-5-(2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 84445-38-5
• 化学式: C₉H₁₅N₂O₁₃P₃
• 分子量: 452.145
• InChiKey: OTXOHOIOFJSIFX-POYBYMJQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.2
• 重原子数：
  27
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  219
• 氢给体数：
  5
• 氢受体数：
  13

反应信息

• 作为反应物：
  描述：

  2-环戊烯-1-羧酸,3-异丙基-1-甲基-(5CI) 在 lithium tetrachloropalladate mercury(II) diacetate 、 sodium chloride 作用下， 生成 [hydroxy-[[(2S,5R)-5-[5-[(E)-3-[[2-[3-(2-hydroxy-6-oxoxanthen-9-yl)propanoyl-methylamino]acetyl]amino]prop-1-enyl]-2,4-dioxopyrimidin-1-yl]oxolan-2-yl]methoxy]phosphoryl] phosphono hydrogen phosphate
  参考文献：
  名称：

  Confalone, Pat. N., Journal of Heterocyclic Chemistry, 1990, vol. 27, # 1, p. 31 - 46

  摘要：

  DOI：
• 作为产物：
  描述：

  、 2′,3′-二脱氧胞苷5′-三磷酸钠盐 、 NaNO2 acetic acid 生成 2-环戊烯-1-羧酸,3-异丙基-1-甲基-(5CI)
  参考文献：
  名称：

  Nucleotides labelled with an infrared dye and their use in nucleic acid detection

  摘要：

  核苷酸-5'-三磷酸和磷酰胺酯，其在碱基部分或磷原子上携带吸收长波长区域的残基，优选为一般式（I）的羧基青黄色素基团；其中R1和R2各表示氢或共同形成苯基残基；如果与核苷酸的连接是通过R4位置，则R3表示氢，如果连接是通过R3位置，则R3表示-NHCS-基团；R4和R5或仅R5表示具有n为3至5的烷基磺酰基团，或者R4代表具有n为3至8的-NHCS-基团，以及使用这些化合物来标记，检测和测序核酸。

  公开号：

  US06573374B1

文献信息

• NUCLEOTIDE ANALOGUES
  申请人：Intelligent Biosystems, Inc.

  公开号：US20170130051A1

  公开（公告）日：2017-05-11

  The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

  本发明提供了利用包含由甲亚砜二硫醚作为可裂解保护基团的3'-O位置封端的脱氧核苷三磷酸的方法、组合物、混合物和试剂盒，以及可逆地连接到所述脱氧核苷的核碱基的可检测标签。这些化合物为未来的测序技术提供了新的可能性，包括但不限于合成测序。
• [EN] URIDINE DI- OR TRI-PHOSPHATE DERIVATIVES AND USES THEREOF<br/>[FR] DÉRIVÉS DU DI- OU TRIPHOSPHATE D'URIDINE ET LEURS UTILISATIONS
  申请人：UNIV BAR ILAN

  公开号：WO2012073237A1

  公开（公告）日：2012-06-07

  The invention provides particular uridine di- and tri-phosphate derivatives, and pharmaceutical compositions thereof. These compounds are useful for treatment of diseases, disorders and conditions modulated by P2Y6 receptors, and particularly for lowering intraocular pressure and thereby treating ocular hypertension and/or glaucoma.

  该发明提供了特定的脲苷二磷酸和三磷酸衍生物，以及其药用组合物。这些化合物可用于治疗受P2Y6受体调节的疾病、紊乱和病况，特别是降低眼内压，从而治疗眼压高和/或青光眼。
• Methods of forming circular nucleic acid probes and uses thereof
  申请人：QIAGEN GmbH

  公开号：US07358047B2

  公开（公告）日：2008-04-15

  The present invention relates generally to a method of amplifying closed circular nucleic acid probes and, more particularly, to a method of amplifying closed circular nucleic acid probes by rolling circle amplification. The method of the present invention is useful in a range of applications involving the detection of nucleic acid sequences such as, but not limited to, the identification of genetic disorders, genetic variants or the presence of microbiological or viral agents.

  本发明通常涉及一种通过滚动环形扩增法扩增闭合环状核酸探针的方法，特别是涉及一种扩增闭合环状核酸探针的方法。本发明的方法在涉及检测核酸序列的各种应用中有用，例如但不限于鉴定遗传疾病、基因变异或微生物或病毒代理的存在。
• Propargyl Substituted Nucleoside Compounds and Methods
  申请人：ROSENBLUM Barnett B.

  公开号：US20120309953A1

  公开（公告）日：2012-12-06

  Disclosed, among other things, are compounds having the structure wherein X comprises a bond or a linker, LABEL comprises at least one detectable label, W 1 taken alone is —H or —OH, W 2 is —OH or a non-extendable moiety, W 3 when taken alone is —H or when taken together with W 1 is —CH 2 —O—, and W 4 is OH, monophosphate, diphosphate, or triphosphate. Also disclosed are labeled polynucleotide compounds and methods of use thereof.

  公开的东西包括以下结构的化合物：其中X包括一个键或一个连接物，LABEL包括至少一个可检测的标记，W1单独为-H或-OH，W2为-OH或不可延伸的基团，W3单独为-H或与W1一起为-CH2-O-，W4为OH、单磷酸、二磷酸或三磷酸。还公开了带标记的多核苷酸化合物及其使用方法。
• High throughput polymorphism screening
  申请人：SMITHKLINE BEECHAM CORPORATION

  公开号：EP1256632A2

  公开（公告）日：2002-11-13

  Methods are provided for determining the identity of a polymorphic nucleotide in a complex mixture of nucleic acids where one or more distinct polymorphisms can be present in the mixture, and multiple polymorphisms can be screened in parallel. Target nucleic acids are amplified using bridge amplification techniques. The detection and identification of the specific polymorphic residue(s) is based on readout methods that utilize the specificity of specific enzymes for complementary DNA sequences. These approaches result in a labeled nucleotide covalently attached to the amplicon, where the identity of the nucleotide is informative of the polymorphic sequence. In one aspect, the readout process uses primer extension protocols, where the specific base incorporated by DNA polymerase is determined by the sequence at the polymorphic site. In another aspect, the identity of a specific base hybridized and ligated to the amplicon is determined by the sequence at the polymorphic site. The polynucleotide to which the label has been attached can be detected in situ, i.e. bound to the solid substrate used for amplification; or can be released and detected.

  本发明提供了确定复杂核酸混合物中多态核苷酸特性的方法，混合物中可能存在一种或多种不同的多态性，可同时筛选多种多态性。使用桥式扩增技术扩增目标核酸。利用特异性酶对互补 DNA 序列的特异性，通过读出方法来检测和识别特定的多态残基。这些方法可将标记的核苷酸共价连接到扩增子上，核苷酸的特性可提供多态序列的信息。一方面，读出过程使用引物延伸协议，DNA 聚合酶结合的特定碱基由多态位点的序列决定。另一方面，与扩增子杂交和连接的特异碱基的身份由多态位点的序列决定。附有标签的多核苷酸可在原位检测，即与用于扩增的固体底物结合；也可释放后检测。