N-[9-[(2R,3R,4S,5R)-5-[2-[[(2R,3S,4R,5R)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxyoxolan-3-yl]methylsulfonyl]ethyl]-3-hydroxy-4-(hydroxymethyl)oxolan-2-yl]-6-[2-(4-nitrophenyl)ethoxy]purin-2-yl]-2-methylpropanamide | 200397-59-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: N-[9-[(2R,3R,4S,5R)-5-[2-[[(2R,3S,4R,5R)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxyoxolan-3-yl]methylsulfonyl]ethyl]-3-hydroxy-4-(hydroxymethyl)oxolan-2-yl]-6-[2-(4-nitrophenyl)ethoxy]purin-2-yl]-2-methylpropanamide
• CAS: 200397-59-7
• 化学式: C₅₁H₆₂N₈O₁₄SSi
• 分子量: 1071.25
• InChiKey: HQAFURFUPFEMGM-SYBZBAHISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.01
• 重原子数：
  75
• 可旋转键数：
  21
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.45
• 拓扑面积：
  308
• 氢给体数：
  5
• 氢受体数：
  17

反应信息

• 作为反应物：
  描述：

  N-[9-[(2R,3R,4S,5R)-5-[2-[[(2R,3S,4R,5R)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxyoxolan-3-yl]methylsulfonyl]ethyl]-3-hydroxy-4-(hydroxymethyl)oxolan-2-yl]-6-[2-(4-nitrophenyl)ethoxy]purin-2-yl]-2-methylpropanamide 在 偶氮二甲酸二异丙酯 、 氨 、 三苯基膦 作用下， 以 四氢呋喃 、 甲醇 为溶剂， 反应 3.25h， 生成 N-[9-[(2R,3R,4S,5R)-5-[2-[[(2R,3S,4R,5R)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxyoxolan-3-yl]methylsulfonyl]ethyl]-3-hydroxy-4-(sulfanylmethyl)oxolan-2-yl]-6-[2-(4-nitrophenyl)ethoxy]purin-2-yl]-2-methylpropanamide
  参考文献：
  名称：

  Synthesis and Biodistribution of a Short Nonionic Oligonucleotide Analogue in Mouse with a Potential to Mimic Peptides

  摘要：

  A nonionic RNA analogue of the sequence r(U(SO2)G(SO2)A(SO2)C) has been synthesized where each bridging phosphate diester is replaced by a dimethylene sulfone unit (rSNA). The rSNA was synthesized in solution from 3',5'-bishomo-beta-ribonucleoside derivatives as building blocks. Full experimemtal procedures are provided, and the product and all synthetic inter mediates are fully characterized. The tetramer is nonionic but highly dipolar due to multiple hydrogen bonding opportunities. It is freely soluble in water only at higher pH's, permitting it to be radiolabeled by exchange of the acidic protons cr. to the sulfones with tritiated water. The tritiated molecule was administered intravenously into the tail vein (2.6 mg/kg) of mice, and its distribution was monitored over 48 h. The rSNA, was widely distributed in the biological tissues, including the brain, and excreted in both the feces and the urine. The accumulation of radioactivity was significantly higher in liver and kidney than in other tissues. Radiolabel was recovered from the urine, analyzed by HPLC, and shown to be intact oligonucleotide sulfone. This is the first bioavailability study on a short nonionic oligonucleotide analogue, a class of molecules with potential biomedical applications.

  DOI：

  10.1021/jm970538o
• 作为产物：
  描述：

  Thioacetic acid S-[(2R,3S,4R,5R)-2-[2-(tert-butyl-diphenyl-silanyloxy)-ethyl]-5-(2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-4-hydroxy-tetrahydro-furan-3-ylmethyl] ester 、 在 Oxone 、 lithium hydroxide 、 sodium acetate 、 caesium carbonate 作用下， 生成 [(2R,3R,4R,5R)-2-[2-[[(2R,3R,4R,5R)-4-acetyloxy-5-(6-benzamidopurin-9-yl)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]oxolan-3-yl]methylsulfonyl]ethyl]-5-(4-benzamido-2-oxopyrimidin-1-yl)-4-benzoyloxyoxolan-3-yl]methyl benzoate 、 N-[9-[(2R,3R,4S,5R)-5-[2-[[(2R,3S,4R,5R)-2-[2-[tert-butyl(diphenyl)silyl]oxyethyl]-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxyoxolan-3-yl]methylsulfonyl]ethyl]-3-hydroxy-4-(hydroxymethyl)oxolan-2-yl]-6-[2-(4-nitrophenyl)ethoxy]purin-2-yl]-2-methylpropanamide
  参考文献：
  名称：

  Synthesis and Biodistribution of a Short Nonionic Oligonucleotide Analogue in Mouse with a Potential to Mimic Peptides

  摘要：

  A nonionic RNA analogue of the sequence r(U(SO2)G(SO2)A(SO2)C) has been synthesized where each bridging phosphate diester is replaced by a dimethylene sulfone unit (rSNA). The rSNA was synthesized in solution from 3',5'-bishomo-beta-ribonucleoside derivatives as building blocks. Full experimemtal procedures are provided, and the product and all synthetic inter mediates are fully characterized. The tetramer is nonionic but highly dipolar due to multiple hydrogen bonding opportunities. It is freely soluble in water only at higher pH's, permitting it to be radiolabeled by exchange of the acidic protons cr. to the sulfones with tritiated water. The tritiated molecule was administered intravenously into the tail vein (2.6 mg/kg) of mice, and its distribution was monitored over 48 h. The rSNA, was widely distributed in the biological tissues, including the brain, and excreted in both the feces and the urine. The accumulation of radioactivity was significantly higher in liver and kidney than in other tissues. Radiolabel was recovered from the urine, analyzed by HPLC, and shown to be intact oligonucleotide sulfone. This is the first bioavailability study on a short nonionic oligonucleotide analogue, a class of molecules with potential biomedical applications.

  DOI：

  10.1021/jm970538o

文献信息

• Synthesis and Biodistribution of a Short Nonionic Oligonucleotide Analogue in Mouse with a Potential to Mimic Peptides
  作者：Bernd Eschgfäller、Marcel König、Franziska Boess、Urs A. Boelsterli、Steven A. Benner

  DOI：10.1021/jm970538o

  日期：1998.1.1

  A nonionic RNA analogue of the sequence r(U(SO2)G(SO2)A(SO2)C) has been synthesized where each bridging phosphate diester is replaced by a dimethylene sulfone unit (rSNA). The rSNA was synthesized in solution from 3',5'-bishomo-beta-ribonucleoside derivatives as building blocks. Full experimemtal procedures are provided, and the product and all synthetic inter mediates are fully characterized. The tetramer is nonionic but highly dipolar due to multiple hydrogen bonding opportunities. It is freely soluble in water only at higher pH's, permitting it to be radiolabeled by exchange of the acidic protons cr. to the sulfones with tritiated water. The tritiated molecule was administered intravenously into the tail vein (2.6 mg/kg) of mice, and its distribution was monitored over 48 h. The rSNA, was widely distributed in the biological tissues, including the brain, and excreted in both the feces and the urine. The accumulation of radioactivity was significantly higher in liver and kidney than in other tissues. Radiolabel was recovered from the urine, analyzed by HPLC, and shown to be intact oligonucleotide sulfone. This is the first bioavailability study on a short nonionic oligonucleotide analogue, a class of molecules with potential biomedical applications.