3,5-二硝基苄氧基胺盐酸盐 | 127312-04-3

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 中文名称: 3,5-二硝基苄氧基胺盐酸盐
• 英文名称: 3,5-Dinitrobenzyloxyamine hydrochloride
• 英文别名: O-[(3,5-dinitrophenyl)methyl]hydroxylamine;hydrochloride
• CAS: 127312-04-3
• 化学式: C7H8ClN3O5
• 分子量: 249.61
• InChiKey: XTEHSFDESUDUCU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.32
• 重原子数：
  16
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  127
• 氢给体数：
  2
• 氢受体数：
  6

文献信息

• Methods for the production of 3-o-deactivated-4'-monophosphoryl lipid A (3D-MLA)
  申请人：CORIXA CORPORATION

  公开号：EP2465937A1

  公开（公告）日：2012-06-20

  Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of a deep rough mutant bacterial strain in a medium; (b) maintaining the culture in stationary phase for at least about 5 hr; (c) harvesting cells from the culture; and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-0-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol% of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt% of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content; and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (mM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.

  本文公开了一种生产脂多糖（LPS）的方法，包括：(a)在培养基中培养深粗糙突变细菌菌株；(b)将培养物维持在静止期至少约5小时；(c)从培养物中收获细胞；(d)从细胞中提取LPS。该方法可生产 LPS，LPS 可用于生产 3-0 脱乙酰化单磷脂 A（3D-MLA），该单磷脂 A 具有至少约 20 摩尔%的六乙酰同系物基团。本文还公开了一种从深度粗糙突变细菌菌株细胞培养物中提取脂多糖（LPS）的方法，包括：(a) 用基本上由至少约 75 wt%的具有 1 至 4 个碳原子的脂肪醇和余量的水组成的溶液提取细胞，从而产生磷脂含量降低的细胞；(b) 用由氯仿和甲醇组成的溶液提取磷脂含量降低的细胞，从而产生 LPS 在氯仿和甲醇中的溶液（mM）。该方法可提供磷脂含量降低的中药中的 LPS 溶液，其生产工艺步骤相对简单且成本低廉。
• Methods for the production of 3-O-deactivated-4'-monophosphoryl lipid a (3D-MLA)
  申请人：Corixa Corporation

  公开号：US20030031684A1

  公开（公告）日：2003-02-13

  Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of a deep rough mutant bacterial strain in a medium; (b) maintaining the culture in stationary phase for at least about 5 hr; (c) harvesting cells from the culture; and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-O-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol % of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt % of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content; and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (CM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.

  本文公开了一种生产脂多糖（LPS）的方法，包括：(a)在培养基中培养深粗糙突变细菌菌株；(b)将培养物维持在静止期至少约5小时；(c)从培养物中收获细胞；(d)从细胞中提取LPS。该方法可生产 LPS，LPS 可用于生产 3-O 脱乙酰化单磷脂 A（3D-MLA），3-O-脱乙酰化单磷脂 A 具有至少约 20 摩尔%的六乙酰同系物基团。 本文还公开了一种从深度粗糙突变细菌菌株细胞培养物中提取脂多糖（LPS）的方法，包括：(a) 用主要由至少约 75 wt % 的具有 1 至 4 个碳原子的脂肪醇和余量的水组成的溶液提取细胞，从而产生磷脂含量降低的细胞；(b) 用由氯仿和甲醇组成的溶液提取磷脂含量降低的细胞，从而产生 LPS 在氯仿和甲醇（CM）中的溶液。这种方法可提供磷脂含量降低的 LPS CM 溶液，其生产工艺相对简单，成本低廉。
• METHODS FOR THE PRODUCTION OF 3-O-DEACYLATED-4'-MONOPHOSPHORYL LIPID A (3D-MLA)
  申请人：CORIXA CORPORATION

  公开号：EP1461418B1

  公开（公告）日：2013-04-24
• Methods for the production of 3-O-deactivated-4'-monophosphoryl lipid A (3D-MLA)
  申请人：CORIXA CORPORATION

  公开号：EP2465937B1

  公开（公告）日：2014-08-13
• METHODS FOR THE PRODUCTION OF 3-O-DEACTIVATED-4'-MONOPHOSPHORYL LIPID A (3D-MLA)
  申请人：Myers R. Kent

  公开号：US20070212758A1

  公开（公告）日：2007-09-13

  Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of deep rough mutant bacterial strain in a medium; (b) maintaining the culture in stationary phase for at least about 5 hr; (c) harvesting cells from the culture; and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-O-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol % of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt % of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content; and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (CM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.