fast red TR | 94-95-1

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: fast red TR
• 英文别名: 5-chloro-toluene-2-diazonium ; chloride；5-Chlor-toluol-2-diazonium; Chlorid；4-Chloro-2-methylbenzene-1-diazonium chloride；4-chloro-2-methylbenzenediazonium;chloride
• CAS: 94-95-1
• 化学式: C₇H₆ClN₂*Cl
• 分子量: 189.044
• InChiKey: PVRAWCMFSQBKGP-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  0.14
• 重原子数：
  11
• 可旋转键数：
  0
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  28.2
• 氢给体数：
  0
• 氢受体数：
  2

反应信息

• 作为反应物：
  描述：

  fast red TR 、 alkaline earth salt of/the/ methylsulfuric acid 生成 4-chloro-2-methylthiophenol
  参考文献：
  名称：

  DE499151

  摘要：

  公开号：

文献信息

• Extraction Spectrophotometric Determination of [(2-Chlorophenyl)methylene]propane Dinitrile with Reactive Dyes of the Stabilized Diazonium Salt Type
  作者：Emil Halámek、Zbyněk Kobliha

  DOI：10.1135/cccc19921221

  日期：——

  A spectrophotometric method has been developed for the determination of [(2-chlorophenyl)methylene]propane dinitrile (CS) stabilized by the diazonium salts Fast Red TR and Fast Blue B after extraction with chloroform. Mass spectrometry, ¹H and ¹³C NMR and elemental analysis confirmed the presence of the hydrazoform of the azo dye formed by reaction with malononitrile, as a product of the alkaline hydrolysis of substance CS, and the diazonium salt Fast Red TR.

  已经开发了一种分光光度法，用于在氯仿萃取后测定由重氮盐Fast Red TR和Fast Blue B稳定的[(2-氯苯基)亚甲基]丙烷二腈（CS）。质谱、¹H和¹³C NMR和元素分析证实了该物质的碱性水解产物和重氮盐Fast Red TR反应生成的偶氮染料的肼酰甲烷形式的存在。
• Method of immunochemical assay
  申请人：WAKO PURE CHEMICAL INDUSTRIES, LTD.

  公开号：EP0231830A2

  公开（公告）日：1987-08-12

  The present invention provides an extremely simple and effective method of a sandwich EIA (enzyme immunoassay) system, or more precisely, a method for the detection of an antigen or an antibody by means of a sandwich enzyme immunoassay system using a combined material of insoluble carrier/antigen/enzyme-labeled antibody or a combined material of insoluble carrier/antigen/enzyme-labeled anti-immunoglobulin-antibody which comprises an insoluble carrier having coated thereon a layer of an antibody or antigen specific to the antigen or antibody to be detected and a layer of an enzyme-labeled antibody or enzyme-labeled anto- immunoglobulin-antibody specific to the antigen or antibody to be detected. According to the method of the present invention, the immunoreaction product comprising fixed antibody/ antigen to be detected/labeled antibody or fixed antigen/ antibody to be detected/labeled antiimmunoglobulin- antibody can be formed on the surface ofthe insoluble carrier by only one incubation and only one washing operation.

  本发明提供了一种极为简便有效的夹心酶免疫分析系统方法，更确切地说，是一种利用不溶性载体/抗原/酶标记抗体的组合材料或不溶性载体/抗原/酶标记抗免疫球蛋白-抗体的组合材料，通过夹心酶免疫分析系统检测抗原或抗体的方法。该系统包括不溶性载体，其上涂有一层待检测抗原或抗体的特异性抗体或抗原，以及一层待检测抗原或抗体的特异性酶标记抗体或酶标记抗免疫球蛋白抗体。根据本发明的方法，只需一次孵育和一次洗涤操作，即可在不溶性载体表面形成由固定抗体/待检测抗原/标记抗体或固定抗原/待检测抗体/标记抗免疫球蛋白-抗体组成的免疫反应产物。
• Method of detecting phosphatase
  申请人：AISIN SEIKI KABUSHIKI KAISHA

  公开号：EP0599270A2

  公开（公告）日：1994-06-01

  An easy operable method of detecting phosphatase which comprises a step for the production of a dye to obtain an azo dye by the reaction of phosphatase in a tissue or cell or on chromosome with a 2'-naphthol AS phosphate, e.g. 3-hydroxy- N-2-biphenyl-2-naphthalenecarboxamide phosphate ester, followed by the reaction between the 2'-naphthol AS phosphate with a diazonium salt; an excitation step for the irradiation of excited light to the azo dye; and a detection step for the detection of fluorescence which is emitted upon irradiation of the excited light. The fluorescence is intense and lasts for a long time.

  一种易于操作的检测磷酸酶的方法，它包括一个生产染料的步骤，通过组织或细胞或 染色体上的磷酸酶与 2'-萘酚 AS 磷酸酯（如 3-羟基-N-2-联苯-2-萘甲酰胺磷酸酯）反 应，得到偶氮染料；一个激发步骤，用于激发组织或细胞或染色体上的磷酸酶。3-hydroxy- N-2-biphenyl-2-naphthalenecarboxamide phosphate ester，然后 2'-naphthol AS phosphate 与重氮盐反应；激发步骤，将激发光照射到偶氮染料上；检测步骤，检测激发光照射后发出的荧光。荧光强度高，持续时间长。
• Biological indicator
  申请人：American Sterilizer Company

  公开号：US10011843B2

  公开（公告）日：2018-07-03

  This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

  本发明涉及一种生物指示剂，其成分包括：由形成孢子的细菌组成的宿主生物；产生指示剂酶的报告基因；调控基因；以及将报告基因和调控基因插入宿主生物的载体；宿主生物的基因组中含有转座遗传因子，用于在调控基因中插入插入序列；插入序列包括转座酶、一对末端倒置重复序列和至少一个用于表达转座酶的开放阅读框。载体可被宿主生物吸收。插入序列可插入调控基因中。宿主生物可进行孢子繁殖以形成生物指示剂。本发明公开了一种使用生物指示剂的工艺和装置。
• Rapid read-out biological indicator
  申请人：Minnesota Mining and Manufacturing Company to 3M Innovative Properties Company

  公开号：US20030077688A1

  公开（公告）日：2003-04-24

  A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

  一种快速确定灭菌周期功效的方法，以及一种适用于执行该方法的指示剂，包括将活性酶源置于灭菌周期中，该酶源的活性与通常用于监测灭菌的微生物的活力相关；在灭菌周期完成后，将酶源与有效量的底物系统孵育，该底物系统能够与任何残留的活性酶发生反应，产生可检测的酶修饰产物。