3-(Chloromethyl)-7-hydroxy-2-benzopyrone | 94279-06-8

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 3-(Chloromethyl)-7-hydroxy-2-benzopyrone
• 英文别名: 3-(chloromethyl)-7-hydroxychromen-2-one
• CAS: 94279-06-8
• 化学式: C₁₀H₇ClO₃
• 分子量: 210.61
• InChiKey: VDQNCLDHHFTJDG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2
• 重原子数：
  14
• 可旋转键数：
  1
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.1
• 拓扑面积：
  46.5
• 氢给体数：
  1
• 氢受体数：
  3

文献信息

• Pyrrolysine synthesis and modification
  申请人：Chan K. Michael

  公开号：US20060183888A1

  公开（公告）日：2006-08-17

  Synthetic L-pyrrolysine of formula I, and derivatives thereof, chemical methods of preparing these amino acids and derivatives, and methods of derivatizing these amino acids after incorporation into a peptide or protein. Also provided are derivatives and methods of preparing derivatives, including addition of substituents at any substitutable position; modifications to the lysine alkyl chain; modifications of the pyrroline ring. Also provided are methods of adding labels to the pyrrolysine or derivative thereof.

  提供公式I的合成L-吡咯氨酸及其衍生物，制备这些氨基酸和衍生物的化学方法，以及将这些氨基酸并入肽或蛋白质后衍生化的方法。还提供了衍生物和制备衍生物的方法，包括在任何可取代位置添加取代基；改变赖氨酸烷基链的结构；改变吡咯环的结构。还提供了将标签添加到吡咯氨酸或其衍生物的方法。
• Charging tRNA with pyrrolysine
  申请人：Chan K. Michael

  公开号：US20060166319A1

  公开（公告）日：2006-07-27

  Provided herein is a method for preparing a cell that, when exposed to the non-canonical amino acid, pyrrolysine or a derivative thereof and transformed or transfected with a polynucleotide comprising an in-frame UAG or TAG codon incorporates the pyrrolysine residue or derivative thereof into the protein or polynucleotide into the protein or polypeptide encoded by the polynucleotide. The method comprises introducing a first polynucleotide comprising a sequence that encodes a protein having pyrrolysyl-tRNA synthetase activity and a second polynucleotide comprising a sequence that encodes a pyrrolysine specific tRNA into the cell and maintining the cell under conditions that permit expression of the first and second polynucleotide. Also provided herein are modified cells produced in accordance with the present method and methods of making peptides, polypeptides, and proteins which utilize such modified cells. Also provide are kits comprising pyrrolysine or a pyrrolysine or both. The kits also provide the first polynucleotide and the second polynucelotide or cells produced in accordance with the present methods or both.

  本发明提供了一种制备细胞的方法，当细胞暴露于非典型氨基酸吡咯赖氨酸或其衍生物并用包含框架内UAG或TAG密码子的多核苷酸转化或转染时，该细胞将吡咯赖氨酸残基或其衍生物结合到蛋白质或多核苷酸中，结合到多核苷酸编码的蛋白质或多肽中。该方法包括将包含编码具有吡咯赖氨酸-tRNA 合成酶活性的蛋白质的序列的第一多核苷酸和包含编码吡咯赖氨酸特异性 tRNA 的序列的第二多核苷酸导入细胞，并在允许第一和第二多核苷酸表达的条件下维持细胞。本文还提供了按照本方法生产的修饰细胞，以及利用这种修饰细胞制造肽、多肽和蛋白质的方法。还提供了包含吡咯烷酮或吡咯烷酮或两者的试剂盒。这些试剂盒还提供第一种多核苷酸和第二种多核苷酸或按照本方法生产的细胞或两者。
• HYDROGEL PARTICLES WITH TUNABLE OPTICAL PROPERTIES AND METHODS FOR USING THE SAME
  申请人：SLINGSHOT BIOSCIENCES

  公开号：US20160258856A1

  公开（公告）日：2016-09-08

  Hydrogel particles and their use in cytometic applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.
• US9714897B2
  申请人：——

  公开号：US9714897B2

  公开（公告）日：2017-07-25