Rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals
申请人:Waters Technologies Corporation
公开号:US10416166B2
公开(公告)日:2019-09-17
Reagents comprising MS active, fluorescent molecules with an activate functionality for reaction with amines useful in rapid tagging of biomolecules such as N-glycans, proteins, peptides, and amino acids, and uses thereof are taught and described. These MS active, fluorescent molecules may have three functional components such as a tertiary amino group or other MS active atom, a highly fluorescent moiety, and a functional group that rapidly reacts with amines.
本文教导并描述了由 MS 活性荧光分子组成的试剂,这些分子具有与胺反应的活化功能,可用于快速标记 N-聚糖、蛋白质、肽和氨基酸等生物大分子及其用途。这些具有 MS 活性的荧光分子可能具有三种功能成分,如三级氨基或其他 MS 活性原子、高荧光分子和可与胺快速反应的功能基团。