2-[2,3-bis[(Z)-octadec-9-enoxy]propoxy-methoxymethoxy]ethyl-trimethylazanium;chloride | 1375484-35-7

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 甘油脂
• 英文名称: 2-[2,3-bis[(Z)-octadec-9-enoxy]propoxy-methoxymethoxy]ethyl-trimethylazanium;chloride
• CAS: 1375484-35-7
• 化学式: C₄₆H₉₂NO₅*Cl
• 分子量: 774.693
• InChiKey: XBWCSQBPBMBUGJ-ZVDJXTMWSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  10.14
• 重原子数：
  53
• 可旋转键数：
  43
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.91
• 拓扑面积：
  46.2
• 氢给体数：
  0
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  2-[methoxy-(1,2-dioleylglyceroxy)methoxy]-N,N,N-trimethylethanammmonium iodide 在 Bio-Rex 5 resin chloride form 作用下， 以 甲醇 、 二氯甲烷 为溶剂， 以100%的产率得到2-[2,3-bis[(Z)-octadec-9-enoxy]propoxy-methoxymethoxy]ethyl-trimethylazanium;chloride
  参考文献：
  名称：

  Novel pH-sensitive cationic lipids with linear ortho ester linkers for gene delivery

  摘要：

  In an effort to develop pH-sensitive lipoplexes for efficient gene delivery, we report three novel cationic lipids containing a linear ortho ester linker that conjugates either the headgroup (Type I) or one hydrocarbon chain (Type II) with the rest of the lipid molecule. The cationic lipids carry either an iodide or a chloride counterion. Compared to our previously reported cyclic ortho ester linker, the linear ortho ester linker facilitated the construction of cationic liposomes and lipoplexes with different helper lipids. The chloride counterion not only facilitated the hydration of the lipid films during liposome construction, but also enhanced the hydrolysis of the ortho ester linker in the lipoplexes. After incubation at endosomal pH 5.5, the Type I lipoplexes aggregated and destabilized the endosome-mimicking model liposomes, but not the Type II lipoplexes. The helper lipids (DOPE or cholesterol) of the lipoplexes enhanced the pH-sensitivity of the Type I lipoplexes. In CV-1 cells (monkey kidney fibroblast), the Type I ortho ester-based lipoplexes, especially those with the chloride counterion, significantly improved the gene transfection efficiency, in some cases by more than 100 fold, compared to their pH-insensitive counterparts consisting of DOTAP. The gene transfection efficiency of the ortho ester-based lipoplexes was well correlated with their rate of aggregation and membrane destabilization in response to the endosomal pH 5.5. (C) 2012 Elsevier Masson SAS. All rights reserved.

  DOI：

  10.1016/j.ejmech.2012.03.013