| 927896-97-7

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• CAS: 927896-97-7
• 化学式: C₁₃H₁₈N₅O₈P
• 分子量: 403.288
• InChiKey: ZKNRLOCMOKOKHW-WOUKDFQISA-L

计算性质

• 辛醇/水分配系数(LogP)：
  -4.14
• 重原子数：
  27.0
• 可旋转键数：
  5.0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.62
• 拓扑面积：
  183.0
• 氢给体数：
  2.0
• 氢受体数：
  12.0

反应信息

• 作为产物：
  描述：

  在 硫酸氢铵 、 recombinant hexahistidine-tagged Caenorhabditis elegans scavenger decapping enzyme 、 水 、 magnesium chloride 作用下， 反应 0.17h， 生成 、 GDP
  参考文献：
  名称：

  Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic studies

  摘要：

  The activity of the Caenorhabditis elegans scavenger decapping enzyme (DcpS) on its natural substrates and dinucleotide cap analogs, modified with regard to the nucleoside base or ribose moiety, has been examined. All tested dinucleotides were specifically cleaved between β‐ and γ‐phosphate groups in the triphosphate chain. The kinetic parameters of enzymatic hydrolysis (Km, Vmax) were determined using fluorescence and HPLC methods, as complementary approaches for the kinetic studies of C. elegans DcpS. From the kinetic data, we determined which parts of the cap structure are crucial for DcpS binding and hydrolysis. We showed that m32,2,7GpppG and m32,2,7GpppA are cleaved with higher rates than their monomethylated counterparts. However, the higher specificity of C. elegans DcpS for monomethylguanosine caps is illustrated by the lower Km values. Modifications of the first transcribed nucleotide did not affect the activity, regardless of the type of purine base. Our findings suggest C. elegans DcpS flexibility in the first transcribed nucleoside‐binding pocket. Moreover, although C. elegans DcpS accommodates bulkier groups in the N7 position (ethyl or benzyl) of the cap, both 2′‐O‐ and 3′‐O‐methylations of 7‐methylguanosine result in a reduction in hydrolysis by two orders of magnitude.

  DOI：

  10.1111/j.1742-4658.2010.07709.x