1-(2,5-dihydroxy-pentyl)-1H-pyrimidine-2,4-dione | 55548-27-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二嗪类
• 英文名称: 1-(2,5-dihydroxy-pentyl)-1H-pyrimidine-2,4-dione
• 英文别名: 1-(2,5-Dihydroxypentyl)pyrimidine-2,4-dione
• CAS: 55548-27-1
• 化学式: C₉H₁₄N₂O₄
• 分子量: 214.221
• InChiKey: LOOLAKSXAQCACN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.4
• 重原子数：
  15
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  89.9
• 氢给体数：
  3
• 氢受体数：
  4

文献信息

• Solid phase sequencing of double-stranded nucleic acids
  申请人：——

  公开号：US20030096258A1

  公开（公告）日：2003-05-22

  Methods for detecting and sequencing of target double-stranded nucleic acid molecules, nucleic acid probes and arrays of probes useful in these methods, and kits and systems that contain these probes aer provided. The methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes include a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments.

  提供了用于检测和测序目标双链核酸分子的方法，用于这些方法的核酸探针和探针阵列，以及包含这些探针的试剂盒和系统。这些方法涉及将与目标互补或同源序列相对应的核酸或核酸的杂交到核酸探针阵列上。这些探针包括单链部分、可选的双链部分和单链部分内的可变序列。可以通过质谱法确定一组杂交核酸的分子量，并从碎片的分子量确定目标的序列。
• SOLID PHASE SEQUENCING OF BIOPOLYMERS
  申请人：Cantor Charles

  公开号：US20110172111A1

  公开（公告）日：2011-07-14

  This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

  本发明涉及检测和测序目标核酸序列的方法，以及在这些方法中有用的质量修饰核酸探针和探针阵列，以及包含这些探针的试剂盒和系统。有用的方法涉及将代表目标互补或同源序列的核酸或核酸杂交到核酸探针阵列上。这些探针包括单链部分，可选的双链部分和单链部分内的可变序列。通过质谱法确定集合的杂交核酸的分子量，并从碎片的分子量确定目标的序列。可以确定序列的核酸包括生物样本中的DNA或RNA，例如患者活检和环境样本。探针可以固定在固体支持上，例如杂交芯片上，以便于自动化的分子量分析和目标序列的鉴定。
• Process for producing optically active hydroxy lactones
  申请人：CHISSO CORPORATION

  公开号：EP0439779A2

  公开（公告）日：1991-08-07

  The invention provides a method for producing an optically active hydroxy lactone which comprises reacting under substantially anhydrous conditions and in the presence of an enzyme an ester and a racemic compound represented by the general formula (I): wherein n is 0 or 1, any one of R¹ and R² is a hydroxy group, and R²=R³=H or R²=R³=CH when R¹ is the hydroxy group, and R¹=R³=H when R² is the hydroxy group to effect a transesterification reaction, and resolving to an optically active alcohol which has R- or S-configuration and the ester of the symmetric alcohol.

  本发明提供了一种生产光学活性羟基内酯的方法，该方法包括在基本无水的条件下和酶的存在下，使酯和通式（I）所代表的外消旋化合物发生反应： 其中n为0或1，R¹和R²中的任一个为羟基，当R¹为羟基时，R²=R³=H或R²=R³=CH，当R²为羟基时，R¹=R³=H，以实现酯交换反应，并解析成具有R-或S-构型的光学活性醇和对称醇的酯。
• Nucleic acid analysis using complete N-mer arrays
  申请人：Affymetrix, Inc. (a California Corporation)

  公开号：EP0995804A2

  公开（公告）日：2000-04-26

  Methods for characterizing mutations in a polynucleotide are provided. Such methods comprise of separately ligating fragments of a reference polynucleotide and a target polynucleotide to identical or substantially identical arrays of polynucleotide probes having single-stranded overhangs constituting complete or substantially complete n-mer sets and comparing their hybridization patterns. The methods can also be used to detect the presence of polymorphisms. Methods are also provided to determine whether two or more polynucleotides of unknown sequences are identical. Further, methods are provided to enumerate and distinguish fragmcnts of polynucleotides based on their terminal sequences. These methods are useful in medicine, pharmacogenomics, biochemistry, and forensic sciences.

  提供了表征多核苷酸突变的方法。这些方法包括将参考多核苷酸和目标多核苷酸的片段分别连接到相同或基本相同的多核苷酸探针阵列上，这些探针具有单链悬垂，构成完整或基本完整的 n-mer集合，并比较它们的杂交模式。这些方法还可用于检测多态性的存在。还提供了确定未知序列的两个或多个多核苷酸是否相同的方法。此外，还提供了根据末端序列列举和区分多核苷酸片段的方法。这些方法可用于医学、药物基因组学、生物化学和法医学。
• Macromolecular arrays on polymeric brushes and methods for preparing the same
  申请人：Affymetrix, Inc.

  公开号：EP1081163A1

  公开（公告）日：2001-03-07

  Polymeric brush substrates and methods for their preparation are provided. Methods are also provided for preparing macromolecular arrays on such polymeric brush substrates. Using polymeric brush substrates allows control over functional site density as well as wettability and porosity of the substrate. These polymeric brushes are useful in solid-phase synthesis of arrays of peptides, polynucleotides or small organic molecules.

  提供了聚合物刷基底及其制备方法。还提供了在这种聚合物刷基底上制备大分子阵列的方法。使用聚合物刷基底可以控制功能位点密度以及基底的润湿性和多孔性。这些聚合物刷可用于肽、多核苷酸或小有机分子阵列的固相合成。