5-{3-(2-Aminoethoxy)propyn-1yl}-2',3'-dideoxycytidine triphosphate | 204062-03-3

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: 5-{3-(2-Aminoethoxy)propyn-1yl}-2',3'-dideoxycytidine triphosphate
• 英文别名: [[(2S,5R)-5-[4-amino-5-[3-(2-aminoethoxy)prop-1-ynyl]-2-oxopyrimidin-1-yl]oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 204062-03-3
• 化学式: C₁₄H₂₃N₄O₁₃P₃
• 分子量: 548.277
• InChiKey: SPJUUAMRIYRHKM-NWDGAFQWSA-N

物化性质

• 密度：
  1.94±0.1 g/cm3 (20 ºC 760 Torr)

计算性质

• 辛醇/水分配系数(LogP)：
  -7.7
• 重原子数：
  34
• 可旋转键数：
  12
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.57
• 拓扑面积：
  263
• 氢给体数：
  6
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  5-{3-(2-trifluoroacetamidoethoxy)propyn-1-yl}-2',3'-dideoxycytidine triphosphate 以 concentrated aqueous NH4 OH 为溶剂， 生成 5-{3-(2-Aminoethoxy)propyn-1yl}-2',3'-dideoxycytidine triphosphate
  参考文献：
  名称：

  Substituted propargylethoxyamido nucleosides, oligonucleotides and

  摘要：

  揭示了具有以下结构的替代丙炔乙氧基氨基核苷：其中X从氨基烷酸、烷基氨基苯甲酸、α-氨基酸和4-氨基-2-丁炔酸组成的群体中选择。R.sub.1和R.sub.2分别选择自--H、低烷基、保护基和标记的群体中；R.sub.3从--H和低烷基组成的群体中选择。B是7-去氮嘌呤、嘌呤或嘧啶核苷碱基。当B是嘌呤或7-去氮嘌呤时，糖基团附加在嘌呤或去氮嘌呤的N.sup.9-位置，当B是嘧啶时，糖基团附加在嘧啶的N.sup.1-位置。当B是嘌呤时，相邻的三键碳附加在嘌呤的8-位置，当B是7-去氮嘌呤时，相邻的三键碳附加在7-去氮嘌呤的7-位置，当B是嘧啶时，相邻的三键碳附加在嘧啶的5-位置。W.sub.1从--H和--OH组成的群体中选择。W.sub.2是--OH或使核苷酸无法在3'-位置形成磷二酯键的基团。W.sub.3从--PO.sub.4、--P.sub.2O.sub.7、--P.sub.3O.sub.10、磷酸盐类似物和--OH组成的群体中选择。此外，提供了一种使用上述X-替代的丙炔乙氧基氨基核苷的引物延伸方法，并提供了包含上述X-替代的丙炔乙氧基氨基核苷的多核苷酸。

  公开号：

  US05770716A1

文献信息

• Fluorescent nucleobase conjugates having anionic linkers
  申请人：PE Corporation (NY)

  公开号：US20020102590A1

  公开（公告）日：2002-08-01

  Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.

  提供了核苷酸染料共轭物和相关化合物，其中染料通过阴离子连接物直接或间接地与核碱基相连。连接物的阴离子性质由连接物中存在的一个或多个阴离子基团提供，例如磷酸酯基、膦酸酯基、磺酸基和羧酸基。当染料作为给体/受体染料对提供时，阴离子连接物可以位于给体和受体之间，或者位于核碱基和给体或受体之间，或两者之间。在一种实施方案中，本发明的共轭物提供了增强的电泳迁移特性，例如，用标记终止子进行二氧基测序的测序片段。
• Propargylethoxyamino nucleotide primer extensions
  申请人：The Perkin-Elmer Corporation

  公开号：US20020045180A1

  公开（公告）日：2002-04-18

  Propargylethoxyamino nucleosides are disclosed having the structure 1 wherein R 1 and R 2 are —H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W 1 is —H or —OH; W 2 is —OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3′-position; and W 3 is —PO 4 , —P 2 O 7 , —P 3 O 10 , phosphate analog, or —OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

  本文披露了具有以下结构的丙炔乙氧氨基核苷：其中R1和R2为—H、低碳基或标记；B为7-脱氮嘌呤、嘌呤或嘧啶核苷碱基；W1为—H或—OH；W2为—OH或一种使核苷酸无法在3'-位点形成磷酸二酯键的基团；W3为—PO4、—P2O7、—P3O10、磷酸盐类似物或—OH。此外，还提供了一种使用上述丙炔乙氧氨基核苷的引物延伸方法。
• Propargylethoxyamino nucleotides
  申请人：PE Corporation (NY)

  公开号：US20030148470A1

  公开（公告）日：2003-08-07

  Propargylethoxyamino nucleosides are disclosed having the structure 1 wherein R 1 and R 2 are —H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W 1 is —H or —OH; W 2 is —OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3′-position; and W 3 is —PO 4 , —P 2 O 7 , —P 3 O 10 , phosphate analog, or —OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

  本文揭示了具有以下结构的丙炔基乙氧基氨基核苷： 其中，R1和R2为—H、低碳基或标记；B为7-去氮嘌呤、嘌呤或嘧啶核苷酸碱基；W1为—H或—OH；W2为—OH或一种使核苷酸无法在3'-位点形成磷酸二酯键的基团；W3为—PO4、—P2O7、—P3O10、磷酸盐类似物或—OH。此外，提供了一种使用上述丙炔基乙氧基氨基核苷的引物延伸方法。
• PROPARGYLETHOXYAMINO NUCLEOTIDES
  申请人：Khan H. Shaheer

  公开号：US20060286561A1

  公开（公告）日：2006-12-21

  Propargylethoxyamino nucleosides are disclosed having the structure wherein R 1 and R 2 are —H lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W 1 is —H or —H; W 2 is —H or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3′-position; and W 3 is —PO 4 , —P 2 O 7 , —P 3 O 10 , phosphate analog, or —OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

  本文揭示了具有以下结构的丙炔基乙氧基氨基核苷： 其中R1和R2是—H低碳基或标记；B是7-去氮嘌呤，嘌呤或嘧啶核苷碱基；W1是—H或—H；W2是—H或使核苷酸在3'-位点无法形成磷酸二酯键的基团；W3是—PO4，—P2O7，—P3O10，磷酸盐类似物或—OH。此外，提供了一种使用上述丙炔基乙氧基氨基核苷的引物延伸方法。
• Fluorescent Nucleobase Conjugates Having Anionic Linkers
  申请人：TAING Meng

  公开号：US20090092986A1

  公开（公告）日：2009-04-09

  Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.

  提供了核苷酸染料共轭物和相关化合物，其中染料通过阴离子连接基团直接或间接地与核碱基相连。连接基团的阴离子特性由存在于连接基团中的一个或多个阴离子基团提供，例如磷酸酯、膦酸酯、磺酸盐和羧酸盐基团。当染料作为给体/受体染料对提供时，阴离子连接基团可以位于给体和受体之间，或位于核碱基和给体或受体之间，或两者之间。在一种实施例中，本发明的共轭物提供了增强的电泳迁移特性，例如用标记的终止子进行二硫代氧核苷酸测序。