function. Therefore, an effective two-photon fluorescent probe with a reasonable two-photon action cross-section to label RNA is now urgently required. In this work, a series of novel two-photon absorbing terpyridine ZnX2 (X = Cl, Br, I) complexes have been designed and an effective RNA imaging probe has been obtained. The results revealed that OTP-ZnCl2 possesses large Stokes shift and two-photon action cross-section
活细胞中RNA的成像是了解细胞内RNA功能的潜在工具。因此,迫切需要一种具有合理的双光子作用截面的有效双光子荧光探针来标记RNA。在这项工作中,已设计了一系列新型的吸收双光子的三联
吡啶ZnX2(X = Cl,Br,I)复合物,并获得了有效的RNA成像探针。结果表明,OTP-ZnCl2具有较大的斯托克斯位移和双光子作用截面。此外,活细胞成像实验表明,OTP-ZnCl2可以通过与核仁RNA结合而染色活细胞中的核仁。通过实验和分子模型计算系统地研究了OTP-ZnCl2选择性核仁染色的机理。由于其细胞毒性低,