DL-5-(3,4-methylenedioxybenzyl)hydantoin | 62682-17-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑烷类
• 英文名称: DL-5-(3,4-methylenedioxybenzyl)hydantoin
• 英文别名: 2,4-Imidazolidinedione, 5-(1,3-benzodioxol-5-ylmethyl)-；5-(1,3-benzodioxol-5-ylmethyl)imidazolidine-2,4-dione
• CAS: 62682-17-1
• 化学式: C₁₁H₁₀N₂O₄
• 分子量: 234.211
• InChiKey: WSZBBVMTNYGMIL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.4
• 重原子数：
  17
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  76.7
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  DL-5-(3,4-methylenedioxybenzyl)hydantoin 在 potassium phosphate buffer 、 Pseudomonas sp. AJ-11220 作用下， 以 various solvent(s) 为溶剂， 反应 2.0h， 生成 (R)-2-氨基-3-(苯并[D][1,3]二氧杂-5-基)丙酸
  参考文献：
  名称：

  Yokozeki, Kenzo; Nakamori, Shigeru; Yamanaka, Shigeru, Agricultural and Biological Chemistry, 1987, vol. 51, # 3, p. 715 - 720

  摘要：

  DOI：
• 作为产物：
  描述：

  5-Piperonyliden-hydantoin 在 sodium hydroxide 、 sodium amalgam 作用下， 生成 DL-5-(3,4-methylenedioxybenzyl)hydantoin
  参考文献：
  名称：

  Deulofeu; Mendive, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1932, vol. 211, p. 1,4

  摘要：

  DOI：

文献信息

• DNA for encoding D-hydantoin hydrolases, DNA for encoding N-carbamyl-D-amino acid hydrolases, recombinant DNA containing the genes, cells transformed with the recombinant DNA, methods for producing proteins utilizing the transformed cells and methods for producing D-amino acids
  申请人：Ajinomoto Co., Inc.

  公开号：US20030113880A1

  公开（公告）日：2003-06-19

  DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.

  编码具有D-咪唑酮酸酶活性的蛋白质的DNA，其碱基序列由序列编号为1的序列清单中的序列表示。编码具有D-氨基甲酰酶活性的蛋白质的DNA，其碱基序列由序列编号为3的序列清单中的序列表示。
• Recombinant D-hydantoin hydrolases and N-carbamyl-D-amino acid hydrolases, and DNA encoding them; uses thereof for producing D-amino acids
  申请人：Ajinomoto Co., Inc.

  公开号：EP1275723A1

  公开（公告）日：2003-01-15

  DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No.1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.

  用于编码具有 D-海因酶活性的蛋白质的 DNA，其碱基序列由序列表中序列 ID No.1 代表。用于编码具有 D-氨基甲酰酶活性的蛋白质的 DNA，其碱基序列由序列表中序列 ID No.
• RECOMBINANT DNA HAVING HYDANTOINASE GENE AND CARBAMYLASE GENE AND PROCESS FOR PRODUCING AMINO ACID
  申请人：Ajinomoto Co., Inc.

  公开号：EP1493809A1

  公开（公告）日：2005-01-05

  The object of the present invention is to prepare a recombinant DNA efficiently co-expressing genes of enzymes such as HHase, and to produce an amino acid efficiently from hydantoin by utilizing such a recombinant DNA. The present invention provides a recombinant DNA having base sequences encoding the following gene regions (I) and (II) incorporated therein in a predetermined direction and in a predetermined order to produce an amino acid efficiently: (I) a gene region containing a base sequence encoding a hydantoinase gene and a trp promoter sequence located upstream of the base sequence encoding the hydantoinase gene regulating expression of the hydantoinase gene, and (II) a gene region containing a base sequence encoding a carbamylase gene and a trp promoter sequence located upstream of the base sequence encoding the carbamylase gene regulating expression of the carbamylase gene.

  本发明的目的是制备一种高效共表达 HHase 等酶基因的重组 DNA，并利用这种重组 DNA 高效地从 hydantoin 中生产氨基酸。本发明提供了一种重组 DNA，其中具有按预定方向和预定顺序结合的编码下列基因区（I）和（II）的碱基序列，以高效生产一种氨基酸： (I)含有编码 hydantoinase 基因的碱基序列和位于编码 hydantoinase 基因的碱基序列上游、调节 hydantoinase 基因表达的 trp 启动子序列的基因区，和 (II) 包含编码氨基甲酰酶基因的碱基序列和位于编码氨基甲酰酶基因的碱基序列上游、调节氨基甲酰酶基因表达的 trp 启动子序列的基因区。
• Mutants of hydantoinase
  申请人：EVONIK TECHNOCHEMIE GMBH

  公开号：US10822627B2

  公开（公告）日：2020-11-03

  The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1. The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.

  本发明涉及一种具有选自(i)或(ii)的氨基酸序列的 hydantoinase，其(i)氨基酸序列选自 SEQ ID NO: 6-20 和 SEQ ID NO: 73-119 (ii)氨基酸序列，其中在 SEQ ID NO: 6-20 和 SEQ ID NO: 73-119 的氨基酸序列中，1 至 75 个氨基酸残基被取代、删除、插入和/或添加，并且该 hydantoinase 的催化活性比具有 SEQ ID NO: 1.2 倍，高于具有 SEQ ID NO: 1 氨基酸序列的 hydantoinase 的催化活性。本发明进一步涉及一种制备氨基酸的工艺，其中使用了所述的水泛素酶。
• DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid
  申请人：AJINOMOTO CO. INC

  公开号：US20030109013A1

  公开（公告）日：2003-06-12

  The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

  本发明提供了一种从5-取代的海因中生产光学活性氨基酸的方法，其方法是从具有上述能力的微细菌属微生物中分离出一种参与将5-取代的海因或N-氨甲酰-L-氨基酸转化为光学活性氨基酸的能力的海因酶基因和N-氨甲酰-L-氨基酸水解酶基因，并通过改进基因扩增和转录及翻译活性从而制备出一种重组体，在该重组体中所产生的所需酶的量有所增加。例如， hydantoinase 基因是编码具有 hydantoinase 活性的蛋白质的 DNA，其核苷酸序列见序列 SEQ ID NO:1 所列。例如，N-氨基甲酰-L-氨基酸水解酶基因是编码具有 N-氨基甲酰-L-氨基酸水解酶活性的蛋白质的 DNA，其核苷酸序列见序列 SEQ ID NO:3。