The invention provides an automated device for accessing the viability of a wide range of organisms based on the metabolic production of fluorescent products from non-fluorescent substrates. Also provide are methods for detecting contaminants in a fluid and measuring the viability of organisms in a fluid or liquid. Components of the invention include the incorporation of a reusable filter to concentrate the organisms, the back flush of the filter to collect the organisms for assay, and the addition of the substrate in a fluorescent detection chamber to detect the enzymatic activity produced by viable organisms to detect the presence of such organisms.
本发明提供了一种自动装置,可根据非荧光底物代谢产生的荧光产物来检测多种
生物的活力。本发明还提供了检测流体中污染物和测量流体或液体中
生物活力的方法。本发明的组成部分包括加入可重复使用的过滤器以浓缩
生物体,过滤器的反冲洗以收集
生物体进行化验,以及在荧光检测室中加入底物以检测存活
生物体产生的酶活性,从而检测此类
生物体的存在。