申请人:Müller Michael
公开号:US20110097766A1
公开(公告)日:2011-04-28
A method is disclosed for forming a covalent bond between two groups having carbonyl radicals in accordance with the present reaction equation:
where
R
1
is hydrogen, a carboxyl group or a C
1
-C
6
alkyl and
R
2
is an aromatic or heteroaromatic radical which may possibly be monosubstituted or disubstituted with halogen and/or a C
1
-C
6
alkyl or is a substituent having the formula -A-B-C-D where A and C are each a C
1
-C
6
alkyl, carbonyl or a covalent bond, B is a heteroatom such as oxygen and sulphur and D is an aliphatic radical having 1 to 6 carbon atoms, or an aromatic or heteroaromatic radical which may possibly be substituted, or where R
1
and R
2
together form a cyclic alkyl radical which may possibly be monosubstituted or disubstituted with a C
1
-C
6
alkyl radical and/or with a hydroxy radical and which may possibly contain a heteroatom such as oxygen or sulphur, and R
3
is hydrogen or a C
1
-C
6
alkyl, wherein the reaction is catalysed by the enzyme YerE.