cephalothin | 481649-28-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 内酰胺类
• 英文名称: cephalothin
• 英文别名: Cephalotin；7,2'-Thienyl-acetamido-cephalosporansaeure；3-acetoxymethyl-7β-(thiophen-2-yl-acetamido)-ceph-3-em-4-carboxylic acid；3-[(Acetyloxy)methyl]-8-oxo-7-[(thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid；3-(acetyloxymethyl)-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
• CAS: 481649-28-9
• 化学式: C₁₆H₁₆N₂O₆S₂
• 分子量: 396.445
• InChiKey: XIURVHNZVLADCM-UHFFFAOYSA-N

物化性质

• 沸点：
  757.2±60.0 °C(Predicted)
• 密度：
  1.56±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -0.4
• 重原子数：
  26
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.38
• 拓扑面积：
  167
• 氢给体数：
  2
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  cephalothin 生成 methyl 2-[[3-(acetyloxymethyl)-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carbonyl]amino]-4-methylsulfanylbutanoate
  参考文献：
  名称：

  TAKEHFUDZI, NARIAKI;NIIMURA, KOITI;ANDO, TAKAO;FUDZII, MASAXIKO;FURUSE, T+

  摘要：

  DOI：
• 作为产物：
  描述：

  cephalothin Na 在 甲烷磺酸 作用下， 以 甲醇 、 二氯甲烷 为溶剂， 反应 0.25h， 生成 cephalothin
  参考文献：
  名称：

  Process for preparing cefoxitin

  摘要：

  公开号：

  EP1748049B1

文献信息

• Optically-Detectable Enzyme Substrates and Their Method of Use
  申请人：Life Technologies Corporation

  公开号：US20160139135A1

  公开（公告）日：2016-05-19

  The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

  本发明涉及一种酶底物化合物，与酶反应后产生可检测的响应，例如光学可检测的响应。具体而言，这些化合物在检测样品中β-内酰胺酶的存在方面具有实用性。除了这些化合物外，还公开了用于分析样品中β-内酰胺酶存在的方法，例如，作为表达包括编码β-内酰胺酶序列的核酸序列的指示器。还公开了包括这些化合物和额外组分的试剂盒，例如细胞、抗体、β-内酰胺酶或使用这些组分进行检测的说明书。
• OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE
  申请人：CORRY Schuyler Boon

  公开号：US20090047692A1

  公开（公告）日：2009-02-19

  The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

  本发明涉及一种酶底物化合物，经过与该酶的反应后提供可检测的响应，例如光学可检测响应。特别是，这些化合物在检测样品中β-内酰胺酶的存在方面具有实用性。除了这些化合物，还揭示了一种分析样品中β-内酰胺酶存在的方法，例如，作为包括编码β-内酰胺酶的核酸序列的表达的指示器。还揭示了包括上述化合物和其他组分的试剂盒，例如细胞、抗体、β-内酰胺酶或使用这些组分进行检测的说明。
• Characterization and engineering of cephalosporin C acylases to produce 7-Aminocephalosporanic acid
  作者：Xiangying Li、Jingang Wang、Wencheng Su、Congcong Li、Ge Qu、Bo Yuan、Zhoutong Sun

  DOI：10.1016/j.mcat.2023.113595

  日期：2023.11

  8-fold increase in specific enzymatic activity (6.1 U/mg) was obtained compared to the wild-type enzyme. The engineered variant also enabled the hydrolysis of a variety of β-lactam antibiotics including Penicillin G and phenylacetyl-7-aminodeacetoxycephalosporanic acid (G-7-ADCA). Overall, this study discovered new CCAs from distinct bacterial origins and enabled the synthesis of various core β-lactam

  7-氨基头孢烷酸(7-ACA)是合成β-内酰胺类抗生素的核心核。头孢菌素 C 酰化酶 (CCA) 是一类能够将头孢菌素 C (CPC) 水解为 7-ACA 的酶。在这项研究中，我们鉴定、表征并设计了新的 CCA 来制备 7-ACA。使用组合活性位点饱和测试（CAST）和迭代进化进行多轮半理性酶设计后获得最佳突变体A14（L159S/A419V）。与野生型酶相比，酶比活性增加了 2.8 倍（6.1 U/mg）。该工程变体还能够水解多种 β-内酰胺抗生素，包括青霉素 G 和苯乙酰基-7-氨基脱乙酰氧基头孢菌酸 (G-7-ADCA)。全面的，
• Advanced drug development and manufacturing
  申请人：Los Alamos National Security, LLC

  公开号：EP2511844A2

  公开（公告）日：2012-10-17

  There is described an apparatus for measuring protein characteristics comprising an X-ray fluorescence (XRF) spectrometer comprising a source of polychromatic X-rays, an X-ray detector, a protein, a molecule that has been exposed to and at least weakly binds to the protein, a plurality of X-ray fluorescence signal data obtained by irradiating chemical elements in the protein and molecule with the polychromatic X-rays and a security system for maintaining records for the data from the plurality of X-ray fluorescence signal measurements. There is also described an x-ray microscope for measuring a sample.

  描述了一种测量蛋白质特性的仪器，该仪器包括一个 X 射线荧光 (XRF) 光谱仪，其中包括一个多色 X 射线源、一个 X 射线探测器、一个蛋白质、一个已暴露于该蛋白质并至少与该蛋白质弱结合的分子、通过用多色 X 射线照射蛋白质和分子中的化学元素而获得的多个 X 射线荧光信号数据，以及一个用于维护多个 X 射线荧光信号测量数据记录的安全系统。此外，还介绍了一种用于测量样品的 X 射线显微镜。
• COMPOSITION FOR HARDENING SOFT TISSUE
  申请人：The Catholic University of Korea Industry-Academic Cooperation Foundation

  公开号：EP3498717A1

  公开（公告）日：2019-06-19

  The present invention relates to various compounds capable of temporarily hardening soft tissue for surgical suturing of the soft tissue. The compounds according to the present invention can temporarily increase the hardness or tension of soft tissue, thereby improving the suturing efficiency during suturing of the soft tissue, thereby preventing aftereffects or the like from occurring due to insufficient anastomosis. In addition, the compounds according to the present invention can temporarily increase the hardness or tension of soft tissue, particularly pancreas, thereby increasing the suturing efficiency during pancreaticoduodenectomy and effectively preventing pancreatic leakage.

  本发明涉及能够暂时硬化软组织的各种化合物，用于软组织的外科缝合。根据本发明的化合物可以暂时增加软组织的硬度或张力，从而在缝合软组织时提高缝合效率，防止因吻合不足而产生后遗症等。此外，根据本发明的化合物可以暂时增加软组织，特别是胰腺的硬度或张力，从而提高胰十二指肠切除术的缝合效率，有效防止胰腺渗漏。