Crowdy; Wain, Annals of Applied Biology, 1951, vol. 38, p. 318,321
作者:Crowdy、Wain
DOI:——
日期:——
Fawcett et al., Annals of Applied Biology, 1955, vol. 43, p. 553,554, 556
作者:Fawcett et al.
DOI:——
日期:——
METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS
申请人:Woody Plant Biotech APS
公开号:EP1217884B1
公开(公告)日:2007-02-21
US6897065B1
申请人:——
公开号:US6897065B1
公开(公告)日:2005-05-24
[EN] METHOD FOR MATURATION OF CONIFER SOMATIC EMBRYOS<br/>[FR] PROCESSUS DE MATURATION D'EMBRYONS SOMATIQUES DE CONIFERES
申请人:FIND JENS IVER
公开号:WO2001020972A1
公开(公告)日:2001-03-29
According to the invention an embryogenic cell mass is cultured with a culture medium comprising an anti-auxin resulting in an unexpected shift in physiology from proliferation to maturation. Proliferation is reduced so that the formation of new immature embryos ceases. Reduction of proliferation facilitates the transition from proliferation to maturation and maturation frequency is increased to a much larger extend than expected. Surprisingly, it has been discovered that the quality of the somatic embryos is not reduced, although the activity of the important endogenous plant growth regulator, auxin, is reduced. As a matter of fact, the overall quality of the mature embryos harvested at the end of maturation is actually increased over the prior art.