reactive blue 2 | 72696-54-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 蒽
• 英文名称: reactive blue 2
• 英文别名: Cibacron Blue；1-amino-4-[4-[[6-chloro-4-(4-sulfoanilino)-1H-1,3,5-triazin-2-ylidene]amino]-3-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid
• CAS: 72696-54-9
• 化学式: C₂₉H₂₀ClN₇O₁₁S₃
• 分子量: 774.169
• InChiKey: IYZXGLGVLYBKAI-UHFFFAOYSA-N

物化性质

• 密度：
  1.845±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  51
• 可旋转键数：
  9
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  323
• 氢给体数：
  7
• 氢受体数：
  18

反应信息

• 作为反应物：
  描述：

  reactive blue 2 在 sodium chloride 作用下， 以 水 为溶剂， 反应 18.0h， 以512 g的产率得到reactive blue 2 (p-isomer)
  参考文献：
  名称：

  [EN] METHOD FOR PURIFICATION OF ANTIBODIES, ANTIBODY FRAGMENTS OR ENGINEERED VARIANTS THEREOF USING SPECIFIC ANTHRAQUINONE DYE-LIGAND STRUCTURES
  [FR] MÉTHODE DE PURIFICATION D'ANTICORPS, DE FRAGMENTS OU DE VARIANTS GÉNÉTIQUEMENT MODIFIÉS DESDITS ANTICORPS, AU MOYEN DE STRUCTURES DE LIGANDS COLORANTS D'ANTHRAQUINONE SPÉCIFIQUES

  摘要：

  本发明涉及一种新型吸附剂，适用于分离或纯化抗体、抗体片段或工程变体的过程，其中包括蒽醌染料配体；相应的纯化过程；以及相应的分析或制备分离试剂盒。

  公开号：

  WO2015117984A1
• 作为产物：
  描述：

  对氨基苯磺酸 在 sodium hydroxide 作用下， 以 水 为溶剂， 反应 18.5h， 生成 reactive blue 2
  参考文献：
  名称：

  [EN] METHOD FOR PURIFICATION OF ANTIBODIES, ANTIBODY FRAGMENTS OR ENGINEERED VARIANTS THEREOF USING SPECIFIC ANTHRAQUINONE DYE-LIGAND STRUCTURES
  [FR] MÉTHODE DE PURIFICATION D'ANTICORPS, DE FRAGMENTS OU DE VARIANTS GÉNÉTIQUEMENT MODIFIÉS DESDITS ANTICORPS, AU MOYEN DE STRUCTURES DE LIGANDS COLORANTS D'ANTHRAQUINONE SPÉCIFIQUES

  摘要：

  本发明涉及一种新型吸附剂，适用于分离或纯化抗体、抗体片段或工程变体的过程，其中包括蒽醌染料配体；相应的纯化过程；以及相应的分析或制备分离试剂盒。

  公开号：

  WO2015117984A1

文献信息

• Method for purification of antibodies, antibody fragments or engineered variants thereof using specific anthraquinone dye-ligand structures
  申请人：BASF SE

  公开号：EP2918641A1

  公开（公告）日：2015-09-16

  The present invention relates to novel adsorbets applicable a process for the separation or purification of antibodies, antibody fragments or engineered variants thereof, which comprise anthraquinone dye ligands; corresponding purification processes; and corresponding analytical or preparative separation kits.

  本发明涉及新型吸附剂，适用于抗体、抗体片段或其工程变体的分离或纯化过程，其中包括蒽醌染料配体；相应的纯化过程；以及相应的分析或制备分离试剂盒。
• Constitutional isomers of Reactive Blue 2 – selective P2Y-receptor antagonists?
  作者：Markus Glänzel、Ralph Bültmann、Klaus Starke、August W Frahm

  DOI：10.1016/s0223-5234(02)01449-6

  日期：2003.3

  The anthraquinone derivative Reactive Blue 2 (RB 2) is one of the most widely used P2-receptor antagonists, still claimed to be P2Y-selective. RB 2 is defined as a mixture of two constitutional isomers and commercially available in different identity and purity. A sample of RB 2, offered for sale by RBI, purchased from Biotrend, Koln, Germany, was chromatographically purified and identified by H-1- and C-13-NMR studies as a 35:65 mixture of the terminal ring F meta and para constitutional isomers. The two constitutional isomers of RB 2 were synthesised and tested alongside with the ortho isomer Cibacron Blue 3GA (CB 3GA) on contractions of the,rat vas deferens (RVD) elicited by alpha,beta-methylene ATP (alpha,beta-MeATP), mediated by P2X(1)-receptors, and relaxations of the carbachol-precontracted guinea pig taenia coli elicited by adenosine 5'-O-(2-thiophosphate) (ADPOS), mediated by P2Y(1)-like-receptors. All compounds inhibited the alpha,beta-MeATP induced contraction of the RVD and the ADPbetaS induced relaxation of the carbachol precontracted guinea-pig taenia coli. The IC50-values at P2X(1)-R were 9.1 muM for CB 3GA, 28.4 muM for RB 2, 19.7 muM for RB 2 meta, and 35.5 muM for RB 2 para. The IC50-values at P2Y(1)-like-R were 17.4 muM for CB 3GA, 7.7 muM for RB 2, 12.0 muM for RB 2 meta, and 2.6 muM for RB 2 para. The results clearly show that neither RB 2 as a mixture nor the pure ortho and meta isomer are P2Y(1)-like- versus P2X(1)-selective antagonists. In contrast the pure para-isomer of RB 2 is a moderately P2Y(1)-like- versus P2X(1)-selective antagonist. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
• METHOD FOR PURIFICATION OF ANTIBODIES, ANTIBODY FRAGMENTS OR ENGINEERED VARIANTS THEREOF USING SPECIFIC ANTHRAQUINONE DYE-LIGAND STRUCTURES
  申请人：BASF SE

  公开号：EP3102637B1

  公开（公告）日：2018-04-11
• METHODS AND COMPOUNDS FOR REGULATING APOPTOSIS
  申请人：Reed John C.

  公开号：US20090118135A1

  公开（公告）日：2009-05-07

  An assay for determining compounds that inhibit activity of a BCl-2 protein, or affect conversion of Bcl-2 from an antiapoptotic to a proapoptotic form are described. In addition, compounds that modulate the function of anti-apoptotic proteins such as Bcl-2 and related Bcl-2 family members are identified.
• [EN] METHOD FOR PURIFICATION OF ANTIBODIES, ANTIBODY FRAGMENTS OR ENGINEERED VARIANTS THEREOF USING SPECIFIC ANTHRAQUINONE DYE-LIGAND STRUCTURES<br/>[FR] MÉTHODE DE PURIFICATION D'ANTICORPS, DE FRAGMENTS OU DE VARIANTS GÉNÉTIQUEMENT MODIFIÉS DESDITS ANTICORPS, AU MOYEN DE STRUCTURES DE LIGANDS COLORANTS D'ANTHRAQUINONE SPÉCIFIQUES
  申请人：BASF SE

  公开号：WO2015117984A1

  公开（公告）日：2015-08-13

  The present invention relates to novel adsorbents applicable a process for the separation or purification of antibodies, antibody fragments or engineered variants thereof, which comprise anthraquinone dye ligands; corresponding purification processes; and corresponding analytical or preparative separation kits.

  本发明涉及一种新型吸附剂，适用于分离或纯化抗体、抗体片段或工程变体的过程，其中包括蒽醌染料配体；相应的纯化过程；以及相应的分析或制备分离试剂盒。