[[3-Azido-5-(4-imino-2-oxo-pyrimidin-1-yl)tetrahydrofuran-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: [[3-Azido-5-(4-imino-2-oxo-pyrimidin-1-yl)tetrahydrofuran-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate
• 英文别名: [[5-(4-amino-2-oxopyrimidin-1-yl)-3-azidooxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• 化学式: C9H15N6O12P3
• 分子量: 492.17
• InChiKey: KTLCZHWMPHUXRA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3.9
• 重原子数：
  30
• 可旋转键数：
  9
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  242
• 氢给体数：
  5
• 氢受体数：
  14

文献信息

• METHOD OF AMPLIFYING MRNAS AND FOR PREPARING FULL LENGTH MRNA LIBRARIES
  申请人：baseclick GmbH

  公开号：EP3842532A1

  公开（公告）日：2021-06-30

  The inventive method for amplifying at least one RNA which is contained in a sample includes reverse transcribing the at least one RNA using a first primer, adding a dideoxy nucleotide which is modified in the 3'-position with a first partner of a pair of azide and alkyne molecules by action of a template-independent polymerase to attach a single 3'-azide- or 3'-alkyne-modified dideoxy nucleotide at the 3'-end of the obtained cDNA, adding an adapter molecule which comprises a polynucleotide sequence and at its 5'-end a second partner of such pair of azide and alkyne molecules and ligating the adapter to the 3'-modified cDNA under formation of a triazole linkage, adding a second primer which is complementary to at least a part of the adapter molecule and which contains at its 3'-end a nucleotide which is complementary to the dideoxy nucleotide at the 3'-end of the cDNA to effect hybridization and binding of the second primer overlapping the triazole linkage, adding a third primer and amplifying the full length cDNA. Uses of such method especially for preparing a full length RNA library and for sequencing of a plurality of RNAs contained in a sample, as well as reagent kits for perfoming such methods are also disclosed and included in the invention..

  本发明用于扩增样品中含有的至少一种 RNA 的方法包括：使用第一引物反转录至少一种 RNA，加入双脱氧核苷酸，该双脱氧核苷酸在 3'位被一对叠氮分子和炔分子的第一伙伴修饰，通过与模板无关的聚合酶的作用，将单个 3'叠氮或 3'炔修饰的双脱氧核苷酸连接到获得的 cDNA 的 3'端、加入一个适配器分子，该分子包含一个多核苷酸序列，在其 5'- 端，是这对叠氮分子和炔分子的第二个伙伴，在形成三唑连接的情况下，将适配器连接到 3'- 修饰的 cDNA 上、加入第二引物，该引物至少与适配器分子的一部分互补，且其 3'- 端含有与 cDNA 3'- 端双脱氧核苷酸互补的核苷酸，以实现第二引物与三唑连接重叠的杂交和结合，加入第三引物并扩增全长 cDNA。 本发明还公开了这种方法的用途，特别是用于制备全长 RNA 文库和对样品中所含的多个 RNA 进行测序，以及用于实施这种方法的试剂盒。