7,15-Dioctoxynonacyclo[18.10.2.22,5.03,16.04,13.06,11.017,31.022,27.028,32]tetratriaconta-1(31),2,4,6(11),7,9,13,15,17,19,22,24,26,28(32),29,33-hexadecaene-12,21-dione

• 分子结构分类: 有机化合物 - 苯类化合物 - 芘
• 英文名称: 7,15-Dioctoxynonacyclo[18.10.2.22,5.03,16.04,13.06,11.017,31.022,27.028,32]tetratriaconta-1(31),2,4,6(11),7,9,13,15,17,19,22,24,26,28(32),29,33-hexadecaene-12,21-dione
• 英文别名: 7,15-dioctoxynonacyclo[18.10.2.22,5.03,16.04,13.06,11.017,31.022,27.028,32]tetratriaconta-1(31),2,4,6(11),7,9,13,15,17,19,22,24,26,28(32),29,33-hexadecaene-12,21-dione
• 化学式: C₅₀H₄₈O₄
• 分子量: 712.9
• InChiKey: YGUDMFJXGYJCHL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  14.8
• 重原子数：
  54
• 可旋转键数：
  16
• 环数：
  9.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  52.6
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• CORE/SHELL-TYPE FLUORESCENT DYE-CONTAINING NANOPARTICLE AND PRODUCTION METHOD OF THE SAME
  申请人：Konica Minolta, Inc.

  公开号：EP3281987A1

  公开（公告）日：2018-02-14

  The present invention provides a method of producing core/shell-type fluorescent dye-containing nanoparticles for immunohistochemical staining or live cell imaging, the method including: the step 1 of polymerizing monomers for thermoplastic resin synthesis in the presence of a fluorescent dye and thereby preparing core particles composed of a thermoplastic resin containing the fluorescent dye; and the step 2 of coating the core particles each with a shell layer composed of a thermosetting resin. By the method of producing fluorescent dye-containing nanoparticles according to the present invention, fluorescent dye-containing nanoparticles having a high brightness, whose dye does not elutes into water, physiological saline, culture medium and the like, can be produced, and the fluorescent dye-containing nanoparticles can be effectively utilized in immunohistochemical staining and live cell imaging.

  本发明提供了一种用于免疫组化染色或活细胞成像的核/壳型含荧光染料纳米颗粒的制备方法，该方法包括：步骤1：在荧光染料存在的情况下，聚合合成热塑性树脂的单体，从而制备由含荧光染料的热塑性树脂组成的核颗粒；步骤2：在每个核颗粒上涂覆由热固性树脂组成的壳层。根据本发明的含荧光染料纳米颗粒的生产方法，可以生产出具有高亮度的含荧光染料纳米颗粒，其染料不会在水、生理盐水、培养基等中洗脱，含荧光染料纳米颗粒可以有效地用于免疫组化染色和活细胞成像。
• PROCESS FOR PRODUCING DYE-CONTAINING HEAT-CURABLE RESIN PARTICLES
  申请人：Konica Minolta, Inc.

  公开号：EP3395906A1

  公开（公告）日：2018-10-31

  The present invention provides a method of producing dye-containing thermosetting resin particles, the method including: dispersing seed particles formed from a thermosetting resin in a dispersion medium; and polymerizing a monomer for thermosetting resin synthesis in the thus obtained dispersion in the presence of a dye and an acid catalyst. By the method of producing dye-containing thermosetting resin particles according to the present invention, dye-containing thermosetting resin particles whose particle size variation coefficient is small at, for example, 8% or less, can be produced without performing a post-treatment such as centrifugation. Therefore, when immunological observation is performed by a fluorescent labeling method using fluorescent dye-containing resin particles produced by the method of producing dye-containing thermosetting resin particles according to the present invention, a high bright spot detection accuracy can be attained.

  本发明提供了一种生产含染料热固性树脂颗粒的方法，该方法包括：在分散介质中分散由热固性树脂形成的种子颗粒；以及在染料和酸催化剂存在下，在由此获得的分散体中聚合用于合成热固性树脂的单体。根据本发明的含染料热固性树脂颗粒的生产方法，无需进行离心等后处理，即可生产出粒度变化系数较小的含染料热固性树脂颗粒，例如粒度变化系数小于 8%。因此，当使用根据本发明的含染料热固性树脂颗粒生产方法生产的含荧光染料树脂颗粒，通过荧光标记方法进行免疫学观察时，可以达到很高的亮点检测精度。
• Method for detecting biological material
  申请人：Konica Minolta, Inc.

  公开号：US10031139B2

  公开（公告）日：2018-07-24

  The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.

  本发明提供了一种染色方法，在这种方法中，即使使用油基装载介质，也不会降低荧光免疫染色标本的荧光染色特性。本发明还提供了一种方法，可防止荧光标签因受激发光照射而变质，并提高通过染色方法获得的荧光免疫染色标本的耐光性。根据本发明的生物物质检测方法是一种从病理标本中特异性检测生物物质的生物物质检测方法，它包括以下步骤：用荧光标签对标本进行免疫染色；固定被染色的标本；使用包括不能与水自由混溶的有机溶剂在内的装载介质装载被固定的标本。在生物物质检测方法中，上述安装介质还包括变色抑制剂。
• Staining agent for staining tissue, production method for staining agent for staining tissue and tissue staining kit including staining agent for staining tissue
  申请人：Konica Minolta, Inc.

  公开号：US10101249B2

  公开（公告）日：2018-10-16

  An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.

  本发明的目的是提供：一种用于组织染色的染色剂，它具有更高的荧光信号评估精度；以及一种包含该染色剂的组织染色试剂盒。用于组织染色的染色剂含有作为染色成分的染料-树脂颗粒，染料-树脂颗粒包括热固性树脂颗粒和固定在树脂颗粒上的荧光染料，其中树脂颗粒含有与荧光染料电荷相反的取代基，并与荧光染料形成离子键或共价键，染料-树脂颗粒的粒度变化系数为 15%或更小。
• Biological substance detection method
  申请人：Konica Minolta, Inc.

  公开号：US10551386B2

  公开（公告）日：2020-02-04

  The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.

  本发明提供了一种从病理标本中特异性检测生物物质的生物物质检测方法，通过该方法，当使用荧光标签进行免疫染色和使用形态观察用染色剂进行形态观察染色同时进行时，即使荧光标签和/或染色剂因激发光照射而变质，也能正确评估荧光观察和免疫染色的结果。根据本发明的生物物质检测方法的特点是，在观察用于免疫染色的荧光标签时，免疫染色部分的亮度保持率与用于形态观察的染色部分的亮度保持率相比在 80% 至 120% 的范围内。