5-甲基脱氧胞苷三磷酸酯 | 22003-12-9

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 5-甲基脱氧胞苷三磷酸酯
• 英文名称: dTTP
• 英文别名: 5-methyl-O^5'-(tetrahydroxy-[1]triphosphoryl)-2'-deoxy-cytidine；5-methyldeoxycytidine 5'-triphosphate；5-methyl-dCTP；[[(2R,3S,5R)-5-(4-amino-5-methyl-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 22003-12-9
• 化学式: C₁₀H₁₈N₃O₁₃P₃
• 分子量: 481.187
• InChiKey: NGYHUCPPLJOZIX-XLPZGREQSA-N

物化性质

• 沸点：
  802.1±75.0 °C(Predicted)
• 密度：
  2.28±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -5.1
• 重原子数：
  29
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  248
• 氢给体数：
  6
• 氢受体数：
  13

反应信息

• 作为反应物：
  描述：

  {5'-d(GCAAGGACCGAAC)}*{3'-d(CGTTCCTGGCTTGGTGTTACTAATC)} 、 5-甲基脱氧胞苷三磷酸酯 生成 {5'-d(GCAAGGACCGAACT)}*{3'-d(CGTTCCTGGCTTGGTGTTACTAATC)}
  参考文献：
  名称：

  摘要：

  DOI：

文献信息

• NUCLEOTIDE ANALOGUES
  申请人：Intelligent Biosystems, Inc.

  公开号：US20170130051A1

  公开（公告）日：2017-05-11

  The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

  本发明提供了利用包含由甲亚砜二硫醚作为可裂解保护基团的3'-O位置封端的脱氧核苷三磷酸的方法、组合物、混合物和试剂盒，以及可逆地连接到所述脱氧核苷的核碱基的可检测标签。这些化合物为未来的测序技术提供了新的可能性，包括但不限于合成测序。
• PROTON ACCEPTOR IMINIUM/CARBOCATION-TYPE COUPLING AGENTS
  申请人：Albericio Fernando

  公开号：US20100144588A1

  公开（公告）日：2010-06-10

  Novel iminium-type coupling agents containing proton acceptors in their iminium moiety, which can be used beneficially as coupling agents in various chemical polypeptide and/or polynucleotide syntheses, and are particularly useful as yield enhancing and racemization suppressing coupling agents for use in peptide syntheses, are disclosed. Further disclosed are a process of preparing such iminium-type coupling agents and their use in the preparation of polypeptides and/or polynucleotides.

  包含质子受体的新型亚胺型偶联剂，可有益地用作各种化学多肽和/或多核苷酸合成中的偶联剂，并且特别适用于在肽合成中提高产率和抑制旋光异构化的偶联剂。还公开了一种制备这种亚胺型偶联剂的方法以及它们在多肽和/或多核苷酸制备中的用途。
• Strand displacement amplification
  申请人：Becton Dickinson and Company

  公开号：EP0497272A1

  公开（公告）日：1992-08-05

  This invention relates a nucleic acid target amplification and detection method which operates at a single temperature and makes use of a polymerase in conjunction with an endonuclease that will nick the polymerized strand such that the polymerase will displace the strand without digestion while generating a newly polymerized strand.

  本发明涉及一种核酸目标扩增和检测方法，该方法在单一温度下操作，利用聚合酶和内切酶，内切酶将聚合链切口，使聚合酶在不消化的情况下置换链，同时产生新的聚合链。
• The use of microfluidic systems in the electrochemical detection of target analytes
  申请人：Clinical Micro Sensors

  公开号：EP1391241A1

  公开（公告）日：2004-02-25

  Figure (1) depicts some preferred embodiments of the invention. Figure (1A) depicts a solid support (5) that has a sample inlet port (10), a first microchannel (15), a storage module (25) (for example, for assay reagents) with a second microchannel (20). The second microchannel (20B), may be in fluid contact directly with the detection module (30) comprising a detection electrode (35), or (20A), a self-assembled monolayer and a binding ligand. Figure (1B) depicts a sample handling well (40) and a second storage well (25A) with a microchannel (20) to the sample handling well (40). For example, the sample handling well (40) could be a cell lysis chamber and the storage well (25A) could contain lysis reagents. Figure (1C) depicts a sample handling well (40) that is a cell capture or enrichment chamber, with an additional reagent storage (25B) for elution buffer. Figure (1D) depicts the addition of a reaction module (45), with a storage module (25C), for example for storage of amplification reagents. Optional waste module (26) is connected to the reaction module (45) via a microchannel (27). All of these embodiments may additionally comprise valves, waste wells, and pumps, including additional electrodes.

  图（1）描述了本发明的一些优选实施例。图 (1A) 描述了一个固体支架 (5)，它有一个样品入口 (10)、一个第一微通道 (15)、一个存储模块 (25)（例如，用于检测试剂）和一个第二微通道 (20)。第二微通道（20B）可直接与包含检测电极（35）的检测模块（30）或（20A）、自组装单层和结合配体进行流体接触。图（1B）描述了一个样品处理井（40）和一个第二存储井（25A），样品处理井（40）上有一个微通道（20）。例如，样品处理井（40）可以是细胞裂解室，而存储井（25A）可以装有裂解试剂。图（1C）描述了一个样品处理井（40），它是一个细胞捕获或富集室，另外还有一个用于洗脱缓冲液的试剂存储井（25B）。图（1D）描述了添加反应模块（45）和存储模块（25C）的情况，例如用于存储扩增试剂。可选的废液模块（26）通过微通道（27）与反应模块（45）相连。所有这些实施方案都可以额外包括阀门、废液井和泵，包括额外的电极。
• Detection of nucleic acid reactions on bead arrays
  申请人：Illumina, Inc.

  公开号：EP1923472A1

  公开（公告）日：2008-05-21

  The present invention provides a method of sequencing a plurality of target nucleic acids each comprising a first domain and an adjacent second domain, in which a plurality of hybridization complexes each comprising a target sequence and a sequencing primer hybridize to the first domain of said target sequence, the hybridization complexes are attached to microspheres,

  本发明提供了一种对多个目标核酸进行测序的方法，每个目标核酸包括一个第一结构域和一个相邻的第二结构域，其中多个杂交复合物各自包括一个目标序列和一个测序引物，与所述目标序列的第一结构域杂交，杂交复合物附着在微球上、