6-[(1-氧代-2-丙烯-1-基)氨基]-己酸 2,5-二氧代-1-吡咯烷基酯 | 63392-86-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡咯烷类
• 中文名称: 6-[(1-氧代-2-丙烯-1-基)氨基]-己酸 2,5-二氧代-1-吡咯烷基酯
• 中文别名: 6-[(1-氧代-2-丙烯-1-基)氨基]-己酸2,5-二氧代-1-吡咯烷基酯
• 英文名称: (2,5-Dioxopyrrolidin-1-yl) 6-(prop-2-enoylamino)hexanoate
• 英文别名: 6-((acryloyl)amino)hexanoic acid, succinimidyl ester；6-((acryloyl)amino)hexanoic acid succinimidyl ester；2,5-Dioxopyrrolidin-1-yl 6-acrylamidohexanoate
• CAS: 63392-86-9
• 化学式: C₁₃H₁₈N₂O₅
• 分子量: 282.296
• InChiKey: AQKLDBRFLGEHCJ-UHFFFAOYSA-N

物化性质

• 密度：
  1.23±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  0.1
• 重原子数：
  20
• 可旋转键数：
  9
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  92.8
• 氢给体数：
  1
• 氢受体数：
  5

安全信息

• 海关编码：
  2925190090

反应信息

• 作为反应物：
  描述：

  6-[(1-氧代-2-丙烯-1-基)氨基]-己酸 2,5-二氧代-1-吡咯烷基酯 在 三乙胺 、 三氟乙酸 作用下， 以 水 、 N,N-二甲基甲酰胺 为溶剂， 反应 5.0h， 生成 N^α-((acryloyl)amino)hexanoyl-L-serine
  参考文献：
  名称：

  蛋白水解聚合物：用氨基酸功能化的聚丙烯酰胺可裂解牛和人血清白蛋白

  摘要：

  合成了具有丝氨酸、天冬氨酸和组氨酸（参与天然丝氨酸蛋白酶胰凝乳蛋白酶催化三联体的氨基酸）的各种组成的聚丙烯酰胺，并研究了它们的蛋白质裂解活性。SDS-PAGE分析表明，一些合成的三元共聚物表现出针对牛和人血清白蛋白的裂解活性。掺入单一类型氨基酸的聚丙烯酰胺也能够裂解蛋白质底物。这些均聚物表现出与 α-胰凝乳蛋白酶不同的独特裂解曲线以及 pH 和温度敏感性。结果表明用氨基酸功能化的聚合物作为蛋白水解人工酶的潜力。

  DOI：

  10.1016/j.bmc.2023.117422
• 作为产物：
  描述：

  6-氨基己酸 在 N,N'-二环己基碳二亚胺 、 sodium hydroxide 作用下， 以 四氢呋喃 、 水 为溶剂， 反应 14.0h， 生成 6-[(1-氧代-2-丙烯-1-基)氨基]-己酸 2,5-二氧代-1-吡咯烷基酯
  参考文献：
  名称：

  Synthesis and Characterization of Barnacle Adhesive Mimetic towards Underwater Adhesion

  摘要：

  合成了一种包含四个丙氨酸单元、羟基和己基的聚丙烯酰胺，作为藤壶水下粘附蛋白的仿生材料。合成的藤壶仿生聚合物溶解于水并与己胺进行缩合反应。通过多个氢键的形成，验证了寡丙氨酸单元的凝胶化。通过拉伸剪切粘附测试，采用胶状藤壶仿生聚合物溶液与聚甲基丙烯酸甲酯（PMMA）板材粘附的粘附强度确认为402 kPa。

  DOI：

  10.1246/cl.150311

文献信息

• EXPANSION MICROSCOPY COMPATIBLE ANCHORABLE H&E STAINING FOR HISTOPATHOLOGY
  申请人：EXPANSION TECHNOLOGIES

  公开号：US20220074827A1

  公开（公告）日：2022-03-10

  The present invention employs the technique for expansion microscopy (E×M) utilizing anchorable derivatives of hematoxylin and eosin (H&E) stained tissue specimens within tissue samples, in order to achieve high resolution detection of biomolecules with precise morphological features of cells and nuclei, which in turn, allows for effective for accurate early stage disease diagnosis including various cancers. Staining with H&E employs anchorable derivatives that can be cross-linked into the E×M hydrogel matrix.

  本发明采用了扩张显微镜技术，利用可锚定血红木和伊红染色（H＆E）染色的组织标本在组织样本中，以实现高分辨率检测具有细胞和核精确形态特征的生物分子，从而实现对包括各种癌症在内的早期疾病诊断的有效准确性。 H＆E染色使用可交联到E×M水凝胶基质中的可锚定衍生物。
• Hydrogels for biomolecule analysis and corresponding method to analyze biomolecules
  申请人：Brueggemeier B. Shawn

  公开号：US20060121535A1

  公开（公告）日：2006-06-08

  Disclosed are a polyacrylamide-based method of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of GST-green fluorescent protein (GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng/mm 2 ) was detectable by direct measurement of GFP fluorescence and this lower detection limit was reduced to 0.080 ng/mm 2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

  本发明涉及一种基于聚丙烯酰胺的表面结合肽和蛋白质阵列制备方法，以及阵列本身和一种使用阵列检测生物分子并测量其浓度、结合亲和力和动力学的方法。肽、蛋白质、融合蛋白、蛋白质复合物、核酸等标记有丙烯酸基团并通过与丙烯酸单体的共聚反应附着于丙烯酸功能化玻璃表面。与非丙烯酸标记的GST-GFP相比，GST-绿色荧光蛋白（GFP）融合蛋白的特异性附着超过了7倍。表面附着的GST-GFP（0.32 ng/mm2）可通过直接测量GFP荧光检测，并且通过间接抗体检测将此较低的检测限降低至0.080 ng/mm2。基于聚丙烯酰胺的表面附着策略也用于测量激酶c-Src对底物磷酸化的动力学。表面附着策略适用于蛋白质组学领域，并解决了与蛋白质微阵列开发相关的变性和脱水问题。
• Copolymers comprising biomolecules
  申请人：Muller Achim

  公开号：US20060148983A1

  公开（公告）日：2006-07-06

  The present invention relates to a copolymer, which is the reaction product of (a) a first prepolymer, comprising a bioactive moiety and at least one radically polymerizable group, and (b) a second prepolymer which is a polyvinyl alcohol having a weight average molecular weight of at least about 2000 that, based on the number of hydroxy groups of the polyvinyl alcohol, comprises from 0.5 to 80% structural units of formula (I) wherein: R is alkylene having up to 8 carbon atoms, R 1 is hydrogen or alkyl having up to seven carbon atoms, and R 2 is an olefinically unsaturated, electron-attracting copolymerizable radical.

  本发明涉及一种共聚物，其是（a）包含生物活性基团和至少一个自由基聚合基团的第一预聚物和（b）是聚乙烯醇的第二预聚物的反应产物，该聚乙烯醇的重均分子量至少为2000，基于聚乙烯醇的羟基数量，包含式（I）的结构单元0.5至80％，其中：R是具有高达8个碳原子的烷基，R1是氢或具有高达七个碳原子的烷基，R2是亲电性不饱和的共聚基团。
• Novel hydrogel copolymer, substrate coated with the copolymer, method of producing microarray using the copolymer, and microarray produced by the method
  申请人：Lee In-ho

  公开号：US20060160120A1

  公开（公告）日：2006-07-20

  A novel hydrogel copolymer, a substrate coated with the copolymer, a method of producing a microarray using the copolymer, and a microarray produced by the method are provided. The use of the hydrogel copolymer makes efficient removal of protein and high integration of nucleic acid and protein on a substrate for a microarray possible.

  提供了一种新型水凝胶共聚物、涂有该共聚物的基板、使用该共聚物制备微阵列的方法，以及由该方法制备的微阵列。使用水凝胶共聚物可以在基板上实现蛋白质的高效去除和核酸和蛋白质的高度整合，从而实现微阵列的制备。
• Protein-acrylamide copolymer hydrogels and method of using same to measure protein concentration and activity
  申请人：Brueggemeier B. Shawn

  公开号：US20070111273A1

  公开（公告）日：2007-05-17

  Disclosed are a polyacrylamide-based method of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect proteins and to measure their concentration, binding affinity, and kinetics. Peptides, proteins, fusion proteins, protein complexes, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of GST-green fluorescent protein (GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng /mm 2 ) was detectable by direct measurement of GFP fluorescence and this lower detection limit was reduced to 0.080 ng /mm 2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

  本发明涉及一种基于聚丙烯酰胺的表面固定肽和蛋白质阵列制备方法、阵列本身以及使用该阵列检测蛋白质并测量其浓度、结合亲和力和动力学的方法。肽、蛋白质、融合蛋白、蛋白质复合物等标记有丙烯酰基并通过与丙烯酸单体的共聚反应连接到丙烯酸功能化玻璃表面。与非丙烯酰标记的GST-GFP相比，GST-绿色荧光蛋白（GFP）融合蛋白的特异性连接增加了7倍以上。表面连接的GST-GFP（0.32 ng/mm2）可以通过直接测量GFP荧光来检测，而这种较低的检测限制可以通过间接抗体检测降低到0.080 ng/mm2。基于聚丙烯酰胺的表面连接策略也用于测量激酶c-Src对底物磷酸化的动力学。该表面连接策略适用于蛋白质组学领域，并解决了与蛋白质微阵列开发相关的变性和脱水问题。