[Hydroxy-[[3-hydroxy-5-(5-iodo-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] phosphono hydrogen phosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: [Hydroxy-[[3-hydroxy-5-(5-iodo-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] phosphono hydrogen phosphate
• 化学式: C₉H₁₄IN₂O₁₄P₃
• 分子量: 594.04
• InChiKey: ZWDWDTXYXXJLJB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.8
• 重原子数：
  29
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  239
• 氢给体数：
  6
• 氢受体数：
  14

文献信息

• NUCLEOSIDE ANALOGUE, PREPARATION METHOD AND APPLICATION
  申请人：GENEMIND BIOSCIENCES COMPANY LIMITED

  公开号：US20200283467A1

  公开（公告）日：2020-09-10

  Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): wherein L 1 , L 2 , and L 3 are each independently a covalent bond or a covalently linked group; B is a base or a base derivative selected from purines, pyrimidines, or analogs thereof; R 1 is —OH, a phosphate group, or a nucleotide; R 2 as H or a cleavable group; R 3 is a detectable group or a targeting group; R 5 is an inhibitory group; R 4 is H, —NH 2 , or —OR 6 , wherein R 6 is H or a cleavable group; and C is a cleavable group or a cleavable bond.

  揭示了具有下面所示结构的核苷酸或核苷酸类似化合物，其制备方法以及在核酸测序中的应用。这些化合物具有公式（I）：其中L1、L2和L3分别独立地是共价键或共价连接的基团；B是从嘌呤、嘧啶或其类似物中选择的碱基或碱基衍生物；R1是—OH、磷酸基团或核苷酸；R2是H或可裂解基团；R3是可检测基团或靶向基团；R5是抑制基团；R4是H、—NH2或—OR6，其中R6是H或可裂解基团；C是可裂解基团或可裂解键。
• Enzymatic nucleic acid synthesis
  申请人：President and Fellows of Harvard College

  公开号：US10745814B2

  公开（公告）日：2020-08-18

  The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.

  本公开提供了活化酶的方法，如易出错或与模板无关的聚合酶，利用电能改变反应区和反应位点的 pH 值，从酶无活性的失活 pH 值变为酶有活性的活化 pH 值，从而将核苷酸添加到引发剂或生长的聚合物链中。然后可将激活 pH 值变回失活 pH 值，并根据需要多次重复该过程，以产生目标核酸序列。
• Nucleoside analogue, preparation method and application
  申请人：GENEMIND BIOSCIENCES COMPANY LIMITED

  公开号：US11512106B2

  公开（公告）日：2022-11-29

  Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): wherein L 1 , L 2 , and L 3 are each independently a covalent bond or a covalently linked group; B is a base or a base derivative selected from purines, pyrimidines, or analogs thereof; R 1 is —OH, a phosphate group, or a nucleotide; R 2 as H or a cleavable group; R 3 is a detectable group or a targeting group; R 5 is an inhibitory group; R 4 is H, —NH 2 , or —OR 6 , wherein R 6 is H or a cleavable group; and C is a cleavable group or a cleavable bond.

  本发明公开了具有下图所示结构的核苷或核苷酸类似物化合物、其制备方法以及在核酸测序中的应用。这些化合物具有式（I）： 其中 L 1 , L 2 和 L 3 各自独立地为共价键或共价连接基团； B 是碱或碱衍生物，选自嘌呤、嘧啶或其类似物； R 1 是-OH、磷酸基团或核苷酸；R 2 为 H 或可裂解基团；R 3 是可检测基团或靶向基团；R 5 是抑制基团 4 是 H、-NH 2 或-OR 6 其中 R 6 是 H 或可裂解基团；C 是可裂解基团或可裂解键。
• Enzymatic Nucleic Acid Synthesis
  申请人：President and Fellows of Harvard College

  公开号：US20190112626A1

  公开（公告）日：2019-04-18

  The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3′ terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3′ terminal nucleotide such that the selected nucleotide becomes a 3′ terminal nucleotide, and repeating the addition step until the polynucleotide is formed.