4-Methyl-benzoic acid 2-oxo-2H-chromen-7-yl ester

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 4-Methyl-benzoic acid 2-oxo-2H-chromen-7-yl ester
• 英文别名: (2-oxochromen-7-yl) 4-methylbenzoate
• 化学式: C₁₇H₁₂O₄
• 分子量: 280.27
• InChiKey: QCVIJCCBBHDKOP-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.7
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.06
• 拓扑面积：
  52.6
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• Screening methods for enzymes and enzyme kits
  申请人：——

  公开号：US20020058254A1

  公开（公告）日：2002-05-16

  Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms. Also disclosed is a process for identifying clones of a recombinant library which express a protein with a desired ctivity by screening a library of expression clones randomly produced from DNA of at least one microorganism, said screeing being effected on expression products of said clones to thereby identify clones which express a protein with a desired activity. Also disclosed is a process of screening clones having DNA from an uncultivatedmicroorganism for a specified protein activity by screening for a specified protein activity in a library of clones prepared by (I) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein activity.

  重组酶库和试剂盒，其中多种酶各自具有不同的物理和/或化学特征，并按共同特征进行分类。这些特征是通过筛选由各种微生物产生的 DNA 文库表达的重组酶来确定的。还公开了一种通过筛选由至少一种微生物的 DNA 随机产生的表达克隆库来确定重组库中表达所需活性蛋白质的克隆的方法，所述筛选是对所述克隆的表达产物进行的，从而确定表达所需活性蛋白质的克隆。本发明还公开了一种筛选克隆的方法，该克隆的 DNA 来自未培养的微生物，筛选方法是：(I) 从至少一种未培养的微生物的 DNA 群体中回收 DNA；(ii) 用回收的 DNA 转化宿主，产生克隆库，筛选克隆库中的特定蛋白质活性。
• SCREENING METHODS FOR ENZYMES AND ENZYME KITS
  申请人：DIVERSA CORPORATION

  公开号：EP0839185B1

  公开（公告）日：2006-11-22