申请人:EASTMAN KODAK COMPANY
(a New Jersey corporation)
公开号:EP0101046A1
公开(公告)日:1984-02-22
The present disclosure relates to methods, compositions and elements for the determination of the amount of lipase in a sample. The method comprises the steps of:
(a) contacting the sample with a reagent composition comprising:
i) a lipase substrate which is a glycerol triester oil having in one of its two a-ester positions a long chain alkyl group having at least 8 carbon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and
ii) an esterase enzyme capable of catalyzing the hydrolysis of a water soluble glycerol diester to glycerol; and
(b) detecting the rate at which glycerol is formed.
The compositions of the present invention include the substrate and enzyme as defined. The element of the invention comprises a support having thereon the described composition. The invention is useful for determining lipase in samples which contain endogenous glycerol, such as blood serum and other body fluids.
本公开涉及测定样品中脂肪酶含量的方法、组合物和元件。该方法包括以下步骤
(a) 将样品与试剂组合物接触,试剂组合物包括
i) 一种脂肪酶底物,它是一种甘油三酯油,在其两个 a 酯位中的一个酯位上具有至少 8 个碳原子的长链烷基,而在其剩余的 两个酯位上具有短链烷基,因此,如果长链烷基被水解,则生成的二酯 是水溶性的;以及
能够催化水溶性甘油二酯水解为甘油的酯酶;以及
(b) 检测甘油形成的速率。
本发明的组合物包括所定义的底物和酶。本发明的元件包括一个支架,其上有所述的组合物。本发明适用于测定含有内源性甘油的样品,如血清和其他体液中的脂肪酶。