Microorganisms detection methods and the separation of agglutinated microorganisms, and a kit for use therein
申请人:E-Y LABORATORIES, INC.
公开号:EP0122023A1
公开(公告)日:1984-10-17
A test for the differential detection of suspected N. gonorrhoeae (N.g.) and N. meningitidis (N.m.) in provided by contacting a suspected sample with a lectin, preferably wheat germ agglutinin and either soy bean agglutinin or STA potato lectin. An agglutinin reaction is a positive indication of the presence of such microorganisms. Separate portions of the specimen may be contaced with fast substrates specific for (a) N.g., (b) N.m., and (c) B. catarrhalis. Suitable fast substrates are proline naphthylamide, gamma glutamyl naphthylamide, and glycyl prolyl naphthylamide, respectively, each substituted with an electron donating group at the 4-position, 6-position, or both. Another portion of the specimen may be reacted with a substrate for beta-galactosidase enzyme, a positive reaction being an indication that the specimen contains N.lactimica. Autoagglutination, which could otherwise give a false positive in the lectin test, may be prevented by pretreatment of the specimen with DNA'ase and/or an anhydride such as succinic anhydride.
一种用于鉴别检测疑似淋球菌(N.g.)和脑膜炎双球菌(N.m.)的检测方法是将疑似样本与凝集素(最好是小麦胚芽凝集素和大豆凝集素或 STA 马铃薯凝集素)接触。凝集素反应是此类微生物存在的阳性指标。标本的不同部分可分别与(a) N.g.、(b) N.m.和(c) B. catarrhalis 的特异性快速底物反应。合适的快速底物分别是脯氨酸萘甲酰胺、γ-谷氨酰萘甲酰胺和甘氨酰脯氨酰萘甲酰胺,每种底物都在 4 位、6 位或两者上被电子捐赠基团取代。标本的另一部分可与β-半乳糖苷酶的底物反应,阳性反应表明标本中含有乳酸奈米卡氏菌。用 DNA'ase 和/或酸酐(如琥珀酸酐)对标本进行预处理,可防止自身凝集,否则会在凝集素检测中出现假阳性。