N-(1-芘甲基)碘乙酰胺 | 153534-76-0

• 分子结构分类: 有机化合物 - 苯类化合物 - 芘
• 中文名称: N-(1-芘甲基)碘乙酰胺
• 英文名称: N-(1-Pyrenemethyl)iodoacetamide
• 英文别名: 2-iodo-N-(pyren-1-ylmethyl)acetamide
• CAS: 153534-76-0
• 化学式: C₁₉H₁₄INO
• 分子量: 399.2
• InChiKey: AYGLXDHPIAFAJF-UHFFFAOYSA-N

物化性质

• 沸点：
  605.7±48.0 °C(Predicted)
• 密度：
  1.691±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  4.8
• 重原子数：
  22
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.11
• 拓扑面积：
  29.1
• 氢给体数：
  1
• 氢受体数：
  1

文献信息

• Labeled oligonucleotides, methods for making same, and compounds useful therefor
  申请人：——

  公开号：US20030208061A1

  公开（公告）日：2003-11-06

  Selectively functionalized oligonucleotides, methods for making same, and compounds useful therefor are disclosed. The oligonucleotides can be selectively functionalized with a first conjugate group at the 3′-terminial position and optionally functionalized with a second conjugate group at the 5′-terminal position and/or one or more internucleotides. Alternatively, the oligonucleotides can be selectively functionalized with a first conjugate group at the 5′-terminal position and optionally functionalized with a second conjugate group at one or more internucleotides. In yet another embodiment, the oligonucleotides can be functionalized with a first conjugate group at one or more internucleotides and with a second conjugate group at one or more different internucleotides.

  选择性功能化寡核苷酸，制备方法以及用于此目的的化合物已被披露。这些寡核苷酸可以在3'-末端位置选择性地与第一共轭基团功能化，并可选择性地在5'-末端位置和/或一个或多个核苷酸之间与第二共轭基团功能化。另外，这些寡核苷酸可以在5'-末端位置选择性地与第一共轭基团功能化，并可选择性地在一个或多个核苷酸之间与第二共轭基团功能化。在另一实施方式中，这些寡核苷酸可以在一个或多个核苷酸之间与第一共轭基团功能化，并在一个或多个不同的核苷酸之间与第二共轭基团功能化。
• PROBE FOR USE IN NUCLEIC ACID ANALYSIS AND DETECTING METHOD
  申请人：The society for Techno-Innovation of Agriculture, Forestry and Fisheries

  公开号：EP0810291A1

  公开（公告）日：1997-12-03

  A probe for detecting nonradioactive nucleic acids by the hybridization technique, and a highly sensitive and highly selective method for the detection. The probe comprises a set of two or more oligonucleotide probes that can hybridize perfectly complementarily with the specific portion of the sequence of the consecutive bases of the target nucleic acid, each oligonucleotide probe being labeled at the 5' or 3' end thereof with a chromophoric group having a suitable spatial arrangement so that adjacent chromophoric groups can yield an excimer or the like when each probe hybridizes with the target nucleic acid. More specifically, although the probe of the invention comprises a set of two or more labeled probes and the labeling groups to be detected are present each on a different probe, the labeling groups take such a spatial arrangement that the two chromophoric groups present on the two probes which have come to exist adjacent to each other only after the hybridization with the target nucleic acid induce unique phenomena such as excimer fluorescence, thereby enabling the target nucleic acid to be detected with a high recognizability. Thus the probe of the invention enables the erroneous recognition which has been problematic heretofore to be reduced remarkably and the types of utilizable labeling groups to be varied widely. Further it is possible to improve the detection sensitivity remarkably because it is possible to reduce the background noise remarkably. In addition, it is possible to discriminate a number of (single-base variation) nucleic acids that are different from one another in only one base present at a specific position to be detected, which has been difficult heretofore.

  一种通过杂交技术检测非放射性核酸的探针，以及一种高灵敏度和高选择性的检测方法。该探针包括一组能与目标核酸的连续碱基序列的特定部分完美互补杂交的两个或多个寡核苷酸探针，每个寡核苷酸探针的 5' 或 3' 端都标记有一个具有适当空间排列的发色基团，这样当每个探针与目标核酸杂交时，相邻的发色基团可以产生一个准分子或类似物。更具体地说，虽然本发明的探针由一组两个或两个以上的标记探针组成，而且要检测的标记基团分别存在于不同的探针上，但标记基团的空间排列方式使存在于两个探针上的两个发色基团仅在与目标核酸杂交后才相邻存在，从而诱发独特的现象，如准分子荧光，从而使目标核酸的检测具有较高的可识别性。因此，本发明的探针能显著减少之前一直存在的错误识别问题，并能使可使用的标记基团的类型多样化。此外，由于可以明显降低背景噪声，因此可以显著提高检测灵敏度。此外，还可以分辨出许多（单碱基变异）核酸，这些核酸彼此不同，只有一个碱基存在于要检测的特定位置，而这在以前是很难做到的。
• Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same
  申请人：Sysmex Corporation

  公开号：EP1233060A2

  公开（公告）日：2002-08-21

  A method for determining the activity of a cell cycle regulatory factor comprising the steps of: preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5'-O-(3-thiotriphosphate) (ATP-γ S) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.

  一种确定细胞周期调节因子活性的方法，包括以下步骤 从活细胞中制备用于测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品； 在样品存在的情况下，使腺苷 5'-O-（3-硫代三磷酸）（ATP-γ S）与细胞周期蛋白依赖性激酶的底物反应，以将单硫代磷酸基团引入底物的丝氨酸或苏氨酸残基中； 通过将标记荧光团或标记酶与引入的单硫代磷酸基团的硫原子偶联，对底物进行标记； 测量标记底物的标记荧光团发出的荧光量，或将标记底物的标记酶与一种物质反应，该物质通过与标记酶反应生成可光学检测的产物，并光学测量生成产物的量；以及 根据测量到的荧光量或测量到的生成物量，参照预先制作的参考曲线，计算细胞周期蛋白依赖性激酶的活性。
• Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same
  申请人：SYSMEX CORPORATION

  公开号：EP1609854A1

  公开（公告）日：2005-12-28

  A method for determining the activity of a cell cycle regulatory factor comprising the steps of: preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5'-O-(3-thiotriphosphate) (ATP-γ S) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.

  一种确定细胞周期调节因子活性的方法，包括以下步骤 从活细胞中制备用于测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品； 在样品存在的情况下，使腺苷 5'-O-（3-硫代三磷酸）（ATP-γ S）与细胞周期蛋白依赖性激酶的底物反应，以将单硫代磷酸基团引入底物的丝氨酸或苏氨酸残基中； 通过将标记荧光团或标记酶与引入的单硫代磷酸基团的硫原子偶联，对底物进行标记； 测量标记底物的标记荧光团发出的荧光量，或将标记底物的标记酶与一种物质反应，该物质通过与标记酶反应生成可光学检测的产物，并光学测量生成产物的量；以及 根据测量到的荧光量或测量到的生成物量，参照预先制作的参考曲线，计算细胞周期蛋白依赖性激酶的活性。
• Method for detecting analyte in sample
  申请人：SYSMEX CORPORATION

  公开号：EP1767943A2

  公开（公告）日：2007-03-28

  A method for detecting an analyte in a sample is described herein. The method comprises: preparing a complex immobilized solid phase comprising a solid phase and a complex on the solid phase; the complex comprising an analyte and a signal emitting substance; treating the complex immobilized solid phase with a blocking agent; and detecting the analyte based on a signal emitted from the signal emitting substance of the complex immobilized solid phase.

  本文描述了一种检测样品中分析物的方法。该方法包括：制备复合物固定固相，复合物固定固相包括固相和固相上的复合物；复合物包括被分析物和信号发射物质；用阻断剂处理复合物固定固相；根据复合物固定固相的信号发射物质发出的信号检测被分析物。