N-(2,4-二氨基-6-氧代-3H-嘧啶-5-基)-N-甲基甲酰胺 | 77440-13-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二嗪类
• 中文名称: N-(2,4-二氨基-6-氧代-3H-嘧啶-5-基)-N-甲基甲酰胺
• 英文名称: 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine
• 英文别名: N-(2,4-Diamino-6-hydroxy-5-pyrimidinyl)-N-methyl-formamid；N-(2,4-diamino-6-oxo-1,6-dihydro-pyrimidin-5-yl)-N-methyl-formamide；N-(2,4-diamino-6-hydroxypyrimidin-5-yl)-N-methylformamide；N-(2,4-diamino-6-oxo-1H-pyrimidin-5-yl)-N-methylformamide
• CAS: 77440-13-2
• 化学式: C₆H₉N₅O₂
• 分子量: 183.17
• InChiKey: CGWDNAFNQOBSCK-UHFFFAOYSA-N

物化性质

• 物理描述：
  Solid

计算性质

• 辛醇/水分配系数(LogP)：
  -2.2
• 重原子数：
  13
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.17
• 拓扑面积：
  114
• 氢给体数：
  3
• 氢受体数：
  3

安全信息

• 海关编码：
  2933599090

反应信息

• 作为产物：
  描述：

  [[(2R,3S,4R,5R)-5-(2-amino-7-methyl-6-oxo-1,8-dihydropurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [[(2R,3S,4R,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] hydrogen phosphate 生成 N-(2,4-二氨基-6-氧代-3H-嘧啶-5-基)-N-甲基甲酰胺
  参考文献：
  名称：

  DARZYNKIEWICZ, EDWARD;STEPINSKI, JANUSZ;TAHARA, STANLEY M.;STOLARSKI, RYS+, NUCLEOSIDES AND NUCLEOTIDES, 9,(1990) N, C. 599-618

  摘要：

  DOI：

文献信息

• Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
  申请人：Ceres Incorporated

  公开号：EP1033405A2

  公开（公告）日：2000-09-06

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. 0Arabidopsis DNA is used in the present experiment, but the procedure is a general one.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体、或对具有共同性状的生物群体进行聚类的工具。 本实验中使用的是拟南芥 DNA，但这是一个通用程序。
• DNA Sequences
  申请人：Ceres Incorporated

  公开号：EP1887081A2

  公开（公告）日：2008-02-13

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体或具有共同性状的生物群体的工具。
• Gel electrophoresis assay system
  申请人：Aarhus Universitet

  公开号：EP2484749A1

  公开（公告）日：2012-08-08

  The present application relates to a gel electrophoresis assay system capable of testing both adherent and non-adherent cells in parallel for different DNA damages. The gel electrophoresis assay system comprises a bottom piece (1), a packing piece (5), an electrophoresis gel anchoring piece (3) positioned in-between the packing piece (5) and the bottom piece (1), and a multi-chamber part (2) positioned on top of the packing piece (5), wherein the multi-chamber part (2) and the bottom piece (1) are fixed by mechanical fixing means (7) so as to provide liquid tight fittings between the multi-chamber part (2), the packing piece (5), the electrophoresis gel anchoring piece (3), and the bottom piece (1), and wherein the multi-chamber part (2) and the packing piece (5) are detachable from the bottom piece (1) and electrophoresis gel anchoring piece (3), respectively, during normal use.

  本申请涉及一种凝胶电泳检测系统，能够同时检测粘附细胞和非粘附细胞的不同 DNA 损伤。凝胶电泳检测系统包括一个底片（1）、一个包装片（5）、一个位于包装片（5）和底片（1）之间的电泳凝胶锚定片（3）以及一个位于包装片（5）顶部的多室部件（2），其中多室部件（2）和底片（1）通过机械固定装置（7）固定，以便在多室部件（2）之间提供液密连接、其中多腔部分（2）和包装件（5）通过机械固定装置（7）固定，以便在多腔部分（2）、包装件（5）、电泳凝胶锚定件（3）和底片（1）之间提供液密配件，并且在正常使用时，多腔部分（2）和包装件（5）可分别与底片（1）和电泳凝胶锚定件（3）分离。
• Identification of genetic modifications
  申请人：WAKE FOREST UNIVERSITY HEALTH SCIENCES

  公开号：US10900071B2

  公开（公告）日：2021-01-26

  Described are methods of detecting modified nucleotide bases in a DNA sample using specific DNA glycosylases to excise target modified bases. DNA molecules are then labeled using a DNA polymerase lacking 3′→5′ exo-nuclease activity and strand displacement activity. The methods can be used to detect epigenetic changes and DNA damage. Provided are methods for diagnosing a disease or condition, determining risk of a disease or condition, identifying appropriate treatment, monitoring effectiveness of treatment, and monitoring side effects of treatment in subjects based on detection of modified bases. Also provided are methods for determining environmental exposure, or an environmental exposure time, of a biological sample containing DNA. Also provided are kits, systems, and devices for performing the described methods.

  描述了利用特定 DNA 糖基化酶切除目标修饰碱基来检测 DNA 样品中修饰核苷酸碱基的方法。然后使用缺乏 3′→5′ 外切核酸酶活性和链置换活性的 DNA 聚合酶标记 DNA 分子。这些方法可用于检测表观遗传变化和 DNA 损伤。所提供的方法可用于诊断疾病或病症、确定疾病或病症的风险、确定适当的治疗方法、监测治疗效果，以及根据对修饰碱基的检测监测治疗对受试者的副作用。还提供了用于确定含有 DNA 的生物样本的环境暴露或环境暴露时间的方法。还提供了用于执行所述方法的试剂盒、系统和装置。
• Nucleic acid binding site assay and selection method
  申请人：——

  公开号：US20020055104A1

  公开（公告）日：2002-05-09

  The invention provides a novel nucleic acid binding assay, which is useful for assessing the sequence-dependence of the binding of ligands to nucleic acid molecules, as well as for determining the affinity of the binding interaction. The invention also provides a selection method based on this nucleic acid binding assay. This selection method allows co-selection of ligands and the nucleic acid molecules that they bind. Additionally, the invention provides kits that can be used for carrying out the methods of the invention.

  本发明提供了一种新型核酸结合测定法，可用于评估配体与核酸分子结合的序列依赖性，以及确定结合相互作用的亲和力。本发明还提供了一种基于这种核酸结合测定的筛选方法。这种选择方法可以共同选择配体及其结合的核酸分子。此外，本发明还提供了可用于实施本发明方法的试剂盒。