N,N-diisopropyl-P-benzylphosphonamidous chloride | 484689-23-8

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: N,N-diisopropyl-P-benzylphosphonamidous chloride
• 英文别名: N-[benzyl(chloro)phosphanyl]-N-propan-2-ylpropan-2-amine
• CAS: 484689-23-8
• 化学式: C₁₃H₂₁ClNP
• 分子量: 257.743
• InChiKey: QKMHGRKGIBPWRD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4
• 重原子数：
  16
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  3.2
• 氢给体数：
  0
• 氢受体数：
  1

反应信息

• 作为反应物：
  描述：

  5'-O-(二甲氧基三苯甲基)-N-异丁酰基-2'-脱氧胞苷 、 N,N-diisopropyl-P-benzylphosphonamidous chloride 生成 2'-deoxy-5'-O-(4,4'-dimethoxytrityl)-N⁴-isobutyrylcytidine 3'-(N,N-diisopropyl-P-benzylphosphonamidite)
  参考文献：
  名称：

  PHENYLALKYL BACKBONE MODIFIED OLIGODEOXYNUCLEOTIDES, THEIR SYNTHESIS AND THE INFLUENCE OF THE ALKYL CHAIN LENGTH

  摘要：

  Phenylalkyl modified phosphoramidites (alkyl chain length n = 1, 2, 3, 5; Fig. 1) were synthesised and incorporated into a DNA hexamer (5'-d(GCCp-GCG); p = place of modification). The obtained diastereomeres were separated by RP-HPLC. After hybridisation with the complementary DNA strand T-m-value and thermodynamic data were measured. The stability of duplexes depends on the linker length and the absolute configuration of the backbone modified oligodeoxynucleotides (Rp, Sp).

  DOI：

  10.1081/ncn-100002535
• 作为产物：
  描述：

  benzyldichlorophosphane 、 二异丙胺 生成 N,N-diisopropyl-P-benzylphosphonamidous chloride
  参考文献：
  名称：

  PHENYLALKYL BACKBONE MODIFIED OLIGODEOXYNUCLEOTIDES, THEIR SYNTHESIS AND THE INFLUENCE OF THE ALKYL CHAIN LENGTH

  摘要：

  Phenylalkyl modified phosphoramidites (alkyl chain length n = 1, 2, 3, 5; Fig. 1) were synthesised and incorporated into a DNA hexamer (5'-d(GCCp-GCG); p = place of modification). The obtained diastereomeres were separated by RP-HPLC. After hybridisation with the complementary DNA strand T-m-value and thermodynamic data were measured. The stability of duplexes depends on the linker length and the absolute configuration of the backbone modified oligodeoxynucleotides (Rp, Sp).

  DOI：

  10.1081/ncn-100002535

文献信息

• Assay for kinases and phosphatases
  申请人：——

  公开号：US20040086954A1

  公开（公告）日：2004-05-06

  Disclosed is a method and corresponding kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.

  本发明公开了一种方法和相应的试剂盒，用于检测选自 EC 2.7.1、EC 3.1.3 和 EC 3.1.4 的酶分类中的酶的存在、活性或两者。该方法一般包括以下步骤：将酶与底物反应足够长的时间，以产生磷酸化或去磷酸化产物；将产物与结合基质接触，使产物附着在基质上；然后分析基质上是否存在固定在基质上的产物、产物的量或产物的存在和量，从而确定酶的存在、活性或酶的存在和活性。
• PHENYLALKYL BACKBONE MODIFIED OLIGODEOXYNUCLEOTIDES, THEIR SYNTHESIS AND THE INFLUENCE OF THE ALKYL CHAIN LENGTH
  作者：Stefan Amberg、Joachim W. Engels

  DOI：10.1081/ncn-100002535

  日期：2001.3.31

  Phenylalkyl modified phosphoramidites (alkyl chain length n = 1, 2, 3, 5; Fig. 1) were synthesised and incorporated into a DNA hexamer (5'-d(GCCp-GCG); p = place of modification). The obtained diastereomeres were separated by RP-HPLC. After hybridisation with the complementary DNA strand T-m-value and thermodynamic data were measured. The stability of duplexes depends on the linker length and the absolute configuration of the backbone modified oligodeoxynucleotides (Rp, Sp).
• ASSAY FOR KINASES AND PHOSPHATASES
  申请人：PROMEGA CORPORATION

  公开号：EP1290213A2

  公开（公告）日：2003-03-12
• US6893834B2
  申请人：——

  公开号：US6893834B2

  公开（公告）日：2005-05-17
• [EN] ASSAY FOR KINASES AND PHOSPHATASES<br/>[FR] DOSAGE POUR KINASES ET PHOSPHATASES
  申请人：PROMEGA CORP

  公开号：WO2001092560A2

  公开（公告）日：2001-12-06

  Disclosed is a method and corresponsind kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.