Disclosed is a method for measurement of the activity of the homocysteinethiolactone hydrolyzing enzyme in a sample, which method comprises mixing and reacting γ-thiobutyrolactone and the sample in the presence of a cholinesterase inhibitor, and measuring 4-mercaptobutyric acid thereby produced. The method is safe and easy in operation, for it does not use a radioactive substrate, and is free of the influence of other compounds occurring in a sample from the body.
本发明公开了一种测量样品中同型半胱
氨酸
硫内酯
水解酶活性的方法,该方法包括在
胆碱酯酶抑制剂存在的情况下,将γ-
硫代
丁酸内酯和样品混合并发生反应,然后测量由此产生的
4-巯基丁酸。该方法不使用放射性底物,不受人体样本中其他化合物的影响,操作安全简便。