申请人:Alfresa Pharma Corporation
公开号:EP1739188A1
公开(公告)日:2007-01-03
Disclosed is a method for measurement of the activity of the homocysteinethiolactone hydrolyzing enzyme in a sample, which method comprises mixing and reacting γ-thiobutyrolactone and the sample in the presence of a cholinesterase inhibitor, and measuring 4-mercaptobutyric acid thereby produced. The method is safe and easy in operation, for it does not use a radioactive substrate, and is free of the influence of other compounds occurring in a sample from the body.
本发明公开了一种测量样品中同型半胱氨酸硫内酯水解酶活性的方法,该方法包括在胆碱酯酶抑制剂存在的情况下,将γ-硫代丁酸内酯和样品混合并发生反应,然后测量由此产生的 4-巯基丁酸。该方法不使用放射性底物,不受人体样本中其他化合物的影响,操作安全简便。