Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
申请人:Spogen Biotech Inc.
公开号:US10407472B2
公开(公告)日:2019-09-10
Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.
提供了含有靶向序列、外孢子体蛋白或外孢子体蛋白片段的融合蛋白,可将融合蛋白靶向蜡样芽孢杆菌家族成员的外孢子体。还提供了表达此类融合蛋白的重组蜡样芽孢杆菌家族成员。还提供了基因失活的蜡样芽孢杆菌家族成员和过度表达外孢子体蛋白的重组蜡样芽孢杆菌家族成员。还提供了涂有重组蜡样芽孢杆菌家族成员的种子和使用重组蜡样芽孢杆菌家族成员的方法(例如,用于刺激植物生长)。还进一步提供了表达融合蛋白的重组蜡样芽孢杆菌家族成员的各种修饰。还提供了由孢子外皮蛋白和感兴趣的蛋白或肽组成的融合蛋白、表达这种融合蛋白的重组细菌、包被这种重组细菌的种子以及使用这种重组细菌的方法(例如用于刺激植物生长)。