N-环己基-N'-(1-芘基)碳二酰亚胺 | 98540-87-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 芘
• 中文名称: N-环己基-N'-(1-芘基)碳二酰亚胺
• 中文别名: 二异癸基苯基磷酸酯
• 英文名称: N-Cyclohexyl-N'-(1-pyrenyl)carbodiimide
• CAS: 98540-87-5
• 化学式: C₂₃H₂₀N₂
• 分子量: 324.4
• InChiKey: GSJAHPSIMGWTCS-UHFFFAOYSA-N

物化性质

• 沸点：
  523.7±23.0 °C(Predicted)
• 密度：
  1.20±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  7.7
• 重原子数：
  25
• 可旋转键数：
  2
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.26
• 拓扑面积：
  24.7
• 氢给体数：
  0
• 氢受体数：
  2

文献信息

• Fret protease assays for clostridial toxins
  申请人：ALLERGAN, INC.

  公开号：EP1901069A1

  公开（公告）日：2008-03-19

  The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a lanthanide donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the lanthanide donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the lanthanide donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the lanthanide donor fluorophore and the acceptor.

  本发明提供的梭菌毒素底物可用于检测任何梭菌毒素的蛋白酶活性，包括所有血清型的肉毒杆菌毒素和破伤风毒素。本发明的梭菌毒素底物包含一个镧系供体荧光团；一个受体，其吸收光谱与镧系供体荧光团的发射光谱重叠；以及一个梭菌毒素识别序列，该序列包括一个裂解位点，裂解位点位于镧系供体荧光团和受体之间，在适当条件下，镧系供体荧光团和受体之间会发生共振能量转移。
• Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides
  申请人：——

  公开号：US20030018169A1

  公开（公告）日：2003-01-23

  The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.

  本发明涉及脂质基质辅助化学键合和合成嵌入脂质基质中的膜多肽的方法和组合物。本发明的实例是通过对未受保护的肽段进行逐步化学选择性连接，生产出嵌入脂质基质中的预折叠膜多肽，其中至少有一个肽段嵌入脂质基质中。可以采用任何适合于未受保护肽段连接的化学选择反应。合适的脂质基质包括脂质体、胶束、细胞膜片和光学各向同性立方脂相基质。根据本发明的方法和组合物合成的预折叠合成和半合成膜多肽还可在特定位点加入一个或多个可检测的分子，如发色团，这些分子可在合成过程中方便地引入。本发明的方法和组合物有多种用途。例如，它们可用于检测配体与膜多肽和包含受体的结构域的结合，因此在结构/功能研究、药物筛选/选择/设计、诊断等方面非常有用，包括高通量应用。本发明的方法和组合物尤其适用于以前无法获得的膜多肽的 FRET 分析。
• Substrates and inhibitors of proteolytic enzymes
  申请人：PEPTIDE THERAPEUTICS LIMITED

  公开号：US20030092067A1

  公开（公告）日：2003-05-15

  The present invention relates to the field of compounds which are substrates or inhibitors of proteolytic enzymes and to apparatus and methods for identifying substrates or inhibitors for proteolytic enzymes. We have devised a combinatorial method for the rapid identification of binding motifs which will greatly expedite the synthesis of inhibitors of a variety of proteolytic enzymes such as aspartyl proteases, serine proteases, metallo proteases and cysteinyl proteases.

  本发明涉及作为蛋白水解酶底物或抑制剂的化合物领域，以及鉴定蛋白水解酶底物或抑制剂的装置和方法。我们设计了一种快速鉴定结合基团的组合方法，这将大大加快天冬氨酰蛋白酶、丝氨酸蛋白酶、金属蛋白酶和半胱氨酰蛋白酶等多种蛋白水解酶抑制剂的合成。
• Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
  申请人：——

  公开号：US20040072270A1

  公开（公告）日：2004-04-15

  The present invention provides a method of determining clostridial toxin activity by (a) contacting with a sample a cell containing a clostridial toxin substrate that includes a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the donor fluorophore and the acceptor, where resonance energy transfer is exhibited between the donor fluorophore and the acceptor under the appropriate conditions; (b) exciting the donor fluorophore; and (c) determining resonance energy transfer of the contacted cell relative to a control cell, where a difference in resonance energy transfer of the contacted cell as compared to the control cell is indicative of clostridial toxin activity.

  本发明提供了一种测定梭状芽孢杆菌毒素活性的方法，其方法是：(a) 将含有梭状芽孢杆菌毒素底物的细胞与样品接触，该底物包括供体荧光团；具有与供体荧光团发射光谱重叠的吸收光谱的受体；以及梭状芽孢杆菌毒素识别序列，该序列含有介于供体荧光团和受体之间的裂解位点；和梭菌毒素识别序列，该序列包含一个介于供体荧光团和受体之间的裂解位点，在适当的条件下，供体荧光团和受体之间表现出共振能量转移；(b) 激发供体荧光团；(c) 确定接触细胞相对于对照细胞的共振能量转移，接触细胞相对于对照细胞的共振能量转移差异表明梭菌毒素的活性。
• SUBSTRATES AND INHIBITORS OF PROTEOLYTIC ENZYMES
  申请人：Medivir UK Ltd

  公开号：EP0906333B1

  公开（公告）日：2001-07-25