(2E)-3-[Methyl(3-methylphenyl)carbamoyl]prop-2-enoic acid

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: (2E)-3-[Methyl(3-methylphenyl)carbamoyl]prop-2-enoic acid
• 英文别名: (E)-4-(N,3-dimethylanilino)-4-oxobut-2-enoic acid
• 化学式: C₁₂H₁₃NO₃
• 分子量: 219.24
• InChiKey: AGXHSFLPIWWWBP-VOTSOKGWSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  16
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.17
• 拓扑面积：
  57.6
• 氢给体数：
  1
• 氢受体数：
  3

文献信息

• USE OF A BIS-MALEIC ANHYDRIDE CROSS-LINKING AGENT FOR FIXATION OF A CELL OR TISSUE SAMPLE
  申请人：Josel Hans-Peter

  公开号：US20120149019A1

  公开（公告）日：2012-06-14

  The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

  本公开涉及新型的双马来酸酐以及令人惊讶的发现，即双马来酸酐交联剂可用于细胞或组织样本的保护/固定。各种双马来酸酐交联剂可用于需要固定细胞或组织样本的方法中。这些试剂和方法在需要去除固定剂以便使用其他试剂进行分析的程序中特别有用。创新的试剂和方法使得使用诸如免疫组织化学、原位荧光杂交、RT-PCR等分析方法更容易可靠地检测各种蛋白质、核酸等。
• Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample
  申请人：Roche Diagnostics GmbH

  公开号：EP2467694B1

  公开（公告）日：2015-01-07
• US8460944B2
  申请人：——

  公开号：US8460944B2

  公开（公告）日：2013-06-11
• US8658109B2
  申请人：——

  公开号：US8658109B2

  公开（公告）日：2014-02-25
• [EN] USE OF A BIS-MALEIC ANHYDRIDE CROSS-LINKING AGENT FOR FIXATION OF A CELL OR TISSUE SAMPLE<br/>[FR] UTILISATION D'UN AGENT DE RÉTICULATION DE TYPE ANHYDRIDE BIS-MALÉIQUE POUR LA FIXATION D'UN ÉCHANTILLON DE CELLULE OU DE TISSU
  申请人：ROCHE DIAGNOSTICS GMBH

  公开号：WO2011020612A1

  公开（公告）日：2011-02-24

  The present invention relates to novel bis-maleic anhydrides. It especially relates to the discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. With great advantage a bis-maleic anhydride cross-linking agent can be used in methods requiring fixation of a cell or tissue sample and at the same time requiring that the fixative has little impact on the later detection of a protein or a nucleic acid in procedures like immunohistochemistry, fluorescence in situ hybridization or RT-PCR.