1-Methyl-4-[2-(4-pyrrolidin-1-ylphenyl)ethenyl]pyridin-1-ium

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡咯烷类
• 英文名称: 1-Methyl-4-[2-(4-pyrrolidin-1-ylphenyl)ethenyl]pyridin-1-ium
• 英文别名: 1-methyl-4-[2-(4-pyrrolidin-1-ylphenyl)ethenyl]pyridin-1-ium
• 化学式: C18H21N2+
• 分子量: 265.4
• InChiKey: VAPIFEJWDAQZSY-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.9
• 重原子数：
  20
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.28
• 拓扑面积：
  7.1
• 氢给体数：
  0
• 氢受体数：
  1

文献信息

• Methods for dye selection for protein melt temperature determinations
  申请人：LIFE TECHNOLOGIES CORPORATION

  公开号：US10024863B2

  公开（公告）日：2018-07-17

  According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

  根据本教导，提供了利用一种或多种荧光团染料进行蛋白质熔体分析的组合物、试剂盒和方法。在某些实施方案中，方法包括通过将至少一种蛋白质与两种或两种以上染料混合来制备样品，并进行受控加热，同时记录样品的荧光发射。这些方法可用于筛选优化蛋白质稳定性的条件、筛选结合并提高蛋白质稳定性的配体（如蛋白质-蛋白质相互作用）、筛选提高稳定性的突变、筛选提高蛋白质稳定性的结晶条件、筛选提高蛋白质稳定性的储存条件，以及筛选提高蛋白质稳定性的蛋白质使用条件（如生产条件、处理条件等）。
• METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS
  申请人：LIFE TECHNOLOGIES CORPORATION

  公开号：US20140315190A1

  公开（公告）日：2014-10-23

  According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.