The present invention provides a protease detection material having, on a support, at least a layer containing a dye precursor and a layer which contains a protease substrate and is disposed on the same side of the support as the layer containing the dye precursor and farther from the support than the layer containing the dye precursor, wherein the layer containing the protease substrate further contains a development center-forming substance. Further, a set of protease detection materials and a method for measuring protease using the above-described protease detection material are provided. A protease detection material, a set of protease detection materials, and a method of measuring protease which allow protease activity to be measured promptly with high sensitivity, and that allow, at the same time, morphological observation of tissues and cells on a thin film to be performed easily.
本发明提供了一种
蛋白酶检测材料,在支撑体上至少具有一层含有
染料前体的层和一层含有
蛋白酶底物的层,该层与含有
染料前体的层位于支撑体的同一侧,且比含有
染料前体的层远离支撑体,其中含有
蛋白酶底物的层进一步含有显影中心形成物质。此外,还提供了一套
蛋白酶检测材料以及使用上述
蛋白酶检测材料测量
蛋白酶的方法。一种
蛋白酶检测材料、一组
蛋白酶检测材料和一种测量
蛋白酶的方法,可快速、高灵敏度地测量
蛋白酶活性,同时可方便地对薄膜上的组织和细胞进行形态观察。