5'-deoxy-5'-N-methyl-N'-(4-[(2-azidoethyl)amino] butyramide)-2',3'-O,O-(1-methylethylidene)adenosine | 1129995-85-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: 5'-deoxy-5'-N-methyl-N'-(4-[(2-azidoethyl)amino] butyramide)-2',3'-O,O-(1-methylethylidene)adenosine
• 英文别名: 4-[[(3aR,4R,6R,6aR)-4-(6-aminopurin-9-yl)-2,2-dimethyl-3a,4,6,6a-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methyl-methylamino]-N-(2-azidoethyl)butanamide
• CAS: 1129995-85-2
• 化学式: C₂₀H₃₀N₁₀O₄
• 分子量: 474.523
• InChiKey: RJLSKJVNYNPURE-BGIGGGFGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.3
• 重原子数：
  34
• 可旋转键数：
  10
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.7
• 拓扑面积：
  144
• 氢给体数：
  2
• 氢受体数：
  11

反应信息

• 作为反应物：
  描述：

  5'-deoxy-5'-N-methyl-N'-(4-[(2-azidoethyl)amino] butyramide)-2',3'-O,O-(1-methylethylidene)adenosine 在 盐酸 、 水 作用下， 反应 0.33h， 以94%的产率得到5'-deoxy-5'-N-methyl-N-(4-[(2-azidoethyl)amino]butyramide)adenosine
  参考文献：
  名称：

  Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. colimethionine repressor, MetJ, for its operator DNA

  摘要：

  本文介绍了一系列稳定的 SAM 拟态物质的高效合成及其促进大肠杆菌蛋氨酸抑制因子 (MetJ) 与其操作 DNA 结合的能力。

  DOI：

  10.1039/b816495a
• 作为产物：
  描述：

  C₂₁H₃₃N₇O₇S 在 sodium azide 作用下， 以 二甲基亚砜 为溶剂， 反应 2.0h， 以188 mg的产率得到5'-deoxy-5'-N-methyl-N'-(4-[(2-azidoethyl)amino] butyramide)-2',3'-O,O-(1-methylethylidene)adenosine
  参考文献：
  名称：

  Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. colimethionine repressor, MetJ, for its operator DNA

  摘要：

  本文介绍了一系列稳定的 SAM 拟态物质的高效合成及其促进大肠杆菌蛋氨酸抑制因子 (MetJ) 与其操作 DNA 结合的能力。

  DOI：

  10.1039/b816495a

文献信息

• Design, synthesis and in vitro evaluation of novel bivalent S-adenosylmethionine analogues
  作者：Catherine Joce、Rebecca White、Peter G. Stockley、Stuart Warriner、W. Bruce Turnbull、Adam Nelson

  DOI：10.1016/j.bmcl.2011.11.017

  日期：2012.1

  In optimal cases, bivalent ligands can bind with exceptionally high affinity to their protein targets. However, designing optimised linkers, that orient the two binding groups perfectly, is challenging, and yet crucial in both fragment-based ligand design and in the discovery of bisubstrate enzyme inhibitors. To further our understanding of linker design, a series of novel bivalent S-adenosylmethionine (SAM) analogues were designed with the aim of interacting with the MetJ dimer in a bivalent sense (1:1 ligand/MetJ dimer). A range of ligands was synthesised and analyzed for ability to promote binding of the Escherichia coli methionine repressor, MetJ, to its operator DNA. Binding of bivalent SAM analogues to the MetJ homodimer in the presence of operator DNA was evaluated by fluorescence anisotropy and the effect of linker length and structure was investigated. The most effective bivalent ligand identified had a flexible linker, and promoted the DNA-protein interaction at 21-times lower concentration than the corresponding monovalent control compound. (C) 2011 Elsevier Ltd. All rights reserved.
• Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. colimethionine repressor, MetJ, for its operator DNA
  作者：Catherine Joce、Jamie Caryl、Peter G. Stockley、Stuart Warriner、Adam Nelson

  DOI：10.1039/b816495a

  日期：——

  The efficient synthesis of a range of stable SAM mimetics, and their ability to promote the binding of the E. coli methionine repressor (MetJ) to its operator DNA, is described.

  本文介绍了一系列稳定的 SAM 拟态物质的高效合成及其促进大肠杆菌蛋氨酸抑制因子 (MetJ) 与其操作 DNA 结合的能力。