Boc-Leu-MFE

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: Boc-Leu-MFE
• 英文别名: (6'-methoxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) (2S)-4-methyl-2-[(2-methylpropan-2-yl)oxycarbonylamino]pentanoate
• 化学式: C₃₂H₃₃NO₈
• 分子量: 559.616
• InChiKey: ISWSRWMYMQRGQB-HKIGIVDHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  6.3
• 重原子数：
  41
• 可旋转键数：
  9
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.34
• 拓扑面积：
  109
• 氢给体数：
  1
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  Boc-Leu-MFE 在 乙二胺四乙酸 、 DL-dithiothreitol 、 cathepsin B 作用下， 以 phosphate buffer 为溶剂， 生成 BOC-L-亮氨酸 、 3-O-methylfluorescein
  参考文献：
  名称：

  Fluorogenic ester substrates to assess proteolytic activity

  摘要：

  The synthesis of a new type of fluorogenic ester substrates is described. Prepared from fluorescein in three steps with common commercially available precursors, they all generate bright green fluorescence upon proteolysis. Their particular structure allows the same substrate be used to report enzymatic activity of various proteases from serine and cysteine superfamilies. The substrate cleavage is sensitive to specific protease inhibitors providing a tool for inhibitor screening. (c) 2006 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2006.06.037
• 作为产物：
  描述：

  methyl 2-(6-methoxy-3-oxo-3H-xanthen-9-yl)benzoate 在 sodium hydroxide 、 N,N'-二环己基碳二亚胺 作用下， 以 甲醇 、 二氯甲烷 为溶剂， 反应 52.0h， 生成 Boc-Leu-MFE
  参考文献：
  名称：

  Fluorogenic ester substrates to assess proteolytic activity

  摘要：

  The synthesis of a new type of fluorogenic ester substrates is described. Prepared from fluorescein in three steps with common commercially available precursors, they all generate bright green fluorescence upon proteolysis. Their particular structure allows the same substrate be used to report enzymatic activity of various proteases from serine and cysteine superfamilies. The substrate cleavage is sensitive to specific protease inhibitors providing a tool for inhibitor screening. (c) 2006 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2006.06.037

文献信息

• Fluorogenic ester substrates to assess proteolytic activity
  作者：Laurent Mugherli、Olga N. Burchak、François Chatelain、Maxim Y. Balakirev

  DOI：10.1016/j.bmcl.2006.06.037

  日期：2006.9

  The synthesis of a new type of fluorogenic ester substrates is described. Prepared from fluorescein in three steps with common commercially available precursors, they all generate bright green fluorescence upon proteolysis. Their particular structure allows the same substrate be used to report enzymatic activity of various proteases from serine and cysteine superfamilies. The substrate cleavage is sensitive to specific protease inhibitors providing a tool for inhibitor screening. (c) 2006 Elsevier Ltd. All rights reserved.