3-(N-ethyl-3,5-dimethylanilino)propane-1-sulfonic acid

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 3-(N-ethyl-3,5-dimethylanilino)propane-1-sulfonic acid
• 化学式: C₁₃H₂₁NO₃S
• 分子量: 271.38
• InChiKey: NPROGRQJOGOVDS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  18
• 可旋转键数：
  6
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  66
• 氢给体数：
  1
• 氢受体数：
  4

文献信息

• Determination method of biological component and reagent kit used therefor
  申请人：——

  公开号：US20020119507A1

  公开（公告）日：2002-08-29

  The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, homocysteine and the like, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteines.

  本发明提供了一种新型的谷胱甘肽依赖性甲醛脱氢酶，它使得通过循环反应定量测量甲醛成为可能，并提供了一种测定甲醛和生物成分（如肌酐、肌酸、同型半胱氨酸等）的方法，该方法产生甲醛作为反应中间体。此外，本发明还提供了用于上述测定方法的试剂盒。本发明提供了一种新型的利用同型半胱氨酸和其他化合物作为一对底物的转移酶测定同型半胱氨酸的方法。特别是，本发明提供了一种同型半胱氨酸的测定方法，其中包括将甜菜碱-同型半胱氨酸甲基转移酶和二甲基甘氨酸氧化酶与样品接触，并测量产生的过氧化氢或甲醛。此外，本发明还提供了一种对巯基化合物稳定的新型二甲基甘氨酸氧化酶，适用于测量。本发明提供了用于上述同型半胱氨酸测定方法的试剂盒。
• METHOD FOR MEASURING TRIGLYCERIDE IN LOW-DENSITY LIPOPROTEIN AND KIT FOR MEASUREMENT
  申请人：Katayama Yuki

  公开号：US20090226944A1

  公开（公告）日：2009-09-10

  The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.

  本发明提供了一种简单准确地测量样品中低密度脂蛋白中甘油三酯的方法，包括按照以下步骤顺序进行：(i)在包括样品和特定表面活性剂（例如聚氧乙烯聚氧烷基多环苯醚）的水介质中，使脂蛋白酯酶作用于样品，生成游离甘油；(ii)去除步骤(i)中反应溶液中存在的游离甘油；(iii)在去除步骤(ii)中的游离甘油的反应溶液中，使脂蛋白酯酶作用于特定表面活性剂（例如聚氧乙烯聚氧烷基烷基醚）的存在下，生成游离甘油；(iv)测量步骤(iii)中生成的游离甘油，以及用于该方法的试剂盒。
• Verfahren und Reagenz zum Nachweis von Schädigungen bzw. Erkrankungen des Herzens
  申请人：BOEHRINGER MANNHEIM GMBH

  公开号：EP0243901A1

  公开（公告）日：1987-11-04

  zum spezifischen und frühen Nachweis von Schädigungen bzw. Erkrankungen des Herzens, insbesondere von akutem Herzinfarkt, wird ein niedermolekularer Metabolit, beispielsweise Creatin oder Creatinphosphat, 2 - 8 Stunden nach dem Auftreten der ersten Symptome bestimmt. Zur Bestimmung des hierbei enzymatisch freigesetzten H202 wird ein Reagenz, das neben einem Elektronen- oder Radikal-Akzeptor ein Anilinderivat als Wasserstoff-Donor beinhaltet, verwendet.

  为了特异性地及早检测心脏损伤或疾病，特别是急性心肌梗塞，可在首发症状出现 2-8 小时后测定低分子量代谢物，如肌酸或磷酸肌酸。 除电子或自由基受体外，还可使用含有苯胺衍生物作为氢供体的试剂来测定在此过程中酶解释放的 H202。
• Method of measuring NADH and NADPH
  申请人：SANWA KAGAKU KENKYUSHO CO., LTD.

  公开号：EP0530732A1

  公开（公告）日：1993-03-10

  There is disclosed a method of measuring NADH or NADPH in a body fluid such as serum for quantitatively measuring a biochemical substance or activity of enzyme therein. According to the method, steps of generating hydrogen peroxide by an action of NADH or NADPH oxidase and measuring the hydrogen peroxide by a chromogenic reaction are concurrently carried out. As the color developing reagent of oxidative coupling reaction, peroxidase, a substance selected from aniline and its derivative, and a substance selected from 3-methyl-2-benzothiazolinone hydrazone and its derivative are employed in combination. If necessary, an enzyme of superoxide dismutase is coexisted in reaction system.

  本发明公开了一种测量血清等体液中 NADH 或 NADPH 的方法，用于定量测量其中的生化物质或酶的活性。根据该方法，通过 NADH 或 NADPH 氧化酶的作用生成过氧化氢和通过显色反应测量过氧化氢的步骤同时进行。作为氧化偶联反应的显色试剂，过氧化物酶、一种选自苯胺及其衍生物的物质和一种选自 3-甲基-2-苯并噻唑啉酮腙及其衍生物的物质被混合使用。必要时，反应体系中还可同时加入超氧化物歧化酶。
• Water-soluble methylenebis(dialkylaniline) derivatives and application thereof
  申请人：DOJINDO LABORATORIES

  公开号：EP0553427A1

  公开（公告）日：1993-08-04

  Novel water-soluble methylenebis(dialkylaniline) derivatives of the following general formula and salts thereof are disclosed. They are useful as dye-forming substances in quantitative determination of peroxides in the copresence of peroxidase or the like.    In the formula, R₁ is hydrogen or a substituent selected from lower alkyl and C₂₋₃ sulfoalkyl or hydroxysulfoalkyl; R₂ is C₂₋₃ sulfoalkyl or hydroxysulfoalkyl; and R₃ is lower alkyl.

  本发明公开了以下通式的新型水溶性亚甲基双（二烷基苯胺）衍生物及其盐类。它们可用作染料形成物质，在过氧化物酶或类似物质的共同作用下定量测定过氧化物。 式中，R₁是氢或选自低级烷基和 C₂₋₃磺酰基或羟基磺酰基的取代基；R₂是 C₂₋₃磺酰基或羟基磺酰基；R₃是低级烷基。