A molecular-level approach has been devised for probing the lateral organization of phospholipid bilayers. This method is based on the equilibration of disulphide-based phospholipid dimers via thiolate-disulphide interchange reactions. Anzlysis of resulting product mixtures defines the tendency of one phhspholipid monomer to become a covalently attached nearest neighbor of another. Investigation of
已经设计了一种分子
水平的方法来探测
磷脂双层的横向组织。该方法基于通过
硫醇盐-二
硫化物交换反应平衡基于二
硫化物的
磷脂二聚体。所得产物混合物的分析定义了一种
磷脂单体成为另一种
磷脂单体共价连接的最近邻的趋势。对源自 1,2-dimyristol-sn-glycero-3-phosphocholine (
DMPC) 和 1,2-dipalmitoyl(sn-glycero-3-phosphocholine (
DPPC) 的二聚体类似物的平衡混合物的研究已经得出了脂质单体确凿的证据在流体相中以及凝胶-流体共存区域中随机分布在整个膜上