A method for identifying unculturable microorganisms in which at least one bacterial cell from an environmental sample containing a plurality of microorganisms is isolated, at least one DNA fragment from the at least one bacterial cell is amplified, cloned into at least one
E. coli
vector and sequenced, resulting in identification of at least one DNA sequence. The at least one DNA sequence is compared with existing DNA databases, resulting in identification of the at least one DNA sequence as derived from either an unculturable microorganism or a known microorganism.
一种鉴定不可培养微
生物的方法,其中从含有多种微
生物的环境样本中分离出至少一个细菌细胞,扩增至少一个细菌细胞中的至少一个 DNA 片段,将其克隆到至少一个
大肠杆菌
载体并进行测序,从而鉴定出至少一个 DNA 序列。将至少一个 DNA 序列与现有的 DNA 数据库进行比较,从而确定至少一个 DNA 序列来自不可培养的微
生物或已知微
生物。